SDS-PAGE and western blotting are traditional technologies in most laboratories working with proteins, in order to separate, visualize and identify some proteins within a mixture. A general detection of all the proteins can be done directly within the electrophoretic gel. The most commonly used protein blotting technique, western blotting, was developed as a result of the need to probe for proteins that were inaccessible to antibodies while in polyacrylamide gels. Western blotting involves the transfer of proteins that have been separated by gel electrophoresis onto a membrane, followed by immunological detection of these proteins. Western blotting combines the resolution of gel electrophoresis with the specificity of immunoassays, allowing individual proteins in mixtures to be identified and analyzed.
In the coming days, we will provide you with some helpful tips and tricks for achieving spectacular western blot results.
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