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Download the Protein Blotting Guide
Download the Stem Cell Guide for Life Science Researchers
:: Posted by American Biotechnologist on 01-16-2014
Like the strings on a violin or the pipes of an organ, the proteins in the human body vibrate in different patterns, scientists have long suspected.
Now, a new study provides what researchers say is the first conclusive evidence that this is true.
Using a technique they developed based on terahertz near-field microscopy, scientists from the University at Buffalo and Hauptman-Woodward Medical Research Institute (HWI) have for the first time observed in detail the vibrations of lysozyme, an antibacterial protein found in many animals.
The team found that the vibrations, which were previously thought to dissipate quickly, actually persist in molecules like the “ringing of a bell,” said UB physics professor Andrea Markelz, PhD, wh0 led the study.
These tiny motions enable proteins to change shape quickly so they can readily bind to other proteins, a process that is necessary for the body to perform critical biological functions like absorbing oxygen, repairing cells and replicating DNA, Markelz said.
The research opens the door to a whole new way of studying the basic cellular processes that enable life.
:: Posted by American Biotechnologist on 09-27-2013
In 1984, César Milstein was awarded the Nobel Prize for developing the hybridoma technique for the production of monoclonal antibodies. The following video by Science Rapper is a tribute to Dr. Milstein in honor of his monumental contribution to science.
:: Posted by American Biotechnologist on 02-25-2013
Researchers at the University of Wisconsin-Madison have found a way to significanlty increase the processing speed at which mass spectrometers identify proteins. Professor Joshua Coon and colleagues from the department of chemistry and biomolecular chemistry, used isotope tags to enable the mass spec to differentiate between as many as 20 different samples at once. The new technology is expected to make mass spec cheaper, faster and more accessible to the scientific masses clamoring to be part of a technique that is on the forefront of biology.
As one astute observer put it:
Proteins are essential building blocks of biology, used in muscle, brain, blood and hormones. If the genes are the blueprints, the proteins patterned on them are the hammers and tongs of life.
With Coon’s new technology, the discovery of the hammers and tongs have life has just been kicked up a notch.
:: Posted by American Biotechnologist on 07-09-2012
Diseases are the result of underlying system interactions NOT simply physical underpinnings. Watch the following talk from Northeastern University Professor Albert-László Barabási to learn how mapping cellular protein reactions can help us discover how diseases work.
:: Posted by American Biotechnologist on 05-22-2012
Power supplies that are used for electrophoresis hold one parameter constant (either voltage, current, or power). The PowerPac™ HC and PowerPac Universal power supplies also have an automatic crossover capability that allows the power supply to switch over to a variable parameter if a set output limit is reached. This helps prevent damage to the transfer cell.
During transfer, if the resistance in the system decreases as a result of Joule heating, the consequences are different and depend on which parameter is held constant.
Transfers Under Constant Voltage
If the voltage is held constant throughout a transfer, the current in most transfer systems increases as the resistance drops due to heating (the exception is most semi-dry systems, where current actually drops as a result of buffer depletion). Therefore, the overall power increases during transfer, and more heating occurs. Despite the increased risk of heating, a constant voltage ensures that field strength remains constant, providing the most efficient transfer possible for tank blotting methods. Use of the cooling elements available with the various tank blotting systems should prevent problems with heating.
Transfers Under Constant Current
If the current is held constant during a run, a decrease in resistance results in a decrease in voltage and power over time. Though heating is minimized, proteins are transferred more slowly due to decreased field strength.
Transfers Under Constant Power
If the power is held constant during a transfer, changes in resistance result in increases in current, but to a lesser degree than when voltage is held constant. Constant power is an alternative to constant current for regulating heat production during transfer.