Posts Tagged ‘PCR’
The polymerase chain reaction (PCR) is a common technique used to amplify, or copy, pieces of DNA. Amplified DNA is then used in genetic analyses for everything from medicine to forensics. In plant research, PCR is a vital step in detecting and sequencing genes, and its applications are endless. However, compounds found in plants often inhibit PCR. Researchers at the University of Southern Mississippi discovered that the use of an additive allows PCR to successfully amplify DNA from once problematic plants.
PCR is widely used in plant sciences but is not 100 percent reliable. Many plant researchers encounter roadblocks when implementing PCR. For example, many plant species contain phenolic compounds that deter herbivores. These compounds are often extracted along with plant DNA and can stop PCR from working.
It’s the little things in life that frustrate me the most. Like fumbling around with a fine tipped Sharpie trying to label a strip of 0.2ml PCR tubes. Now that’s frustrating. Then there are people that use their brains for finding creative solutions instead of just whining about the problem to their lab mates. Here is one such example. I wish I had thought of that!
What tips do you have for your fellow biotechnologists that can help save them both time and sanity?
Several years ago, Bio-Rad Laboratories released the highly successful music video “The PCR Song.” The song, which boasts great lyrics and a catchy tune, went viral receiving over 1 Million views since its release. There have been many attempts to copy Bio-Rad’s success and hundreds of knock-off videos have been posted to YouTube. One thing is for sure, The PCR Song has helped spread awareness about PCR technology among the lay public and is inspiring a whole new generation to learn more about cutting edge science.
Here is the latest PCR Song remix posted by budding future scientists:
Here’s the original video: