In our last post we told you about how Bio-Rad Laboratories very own Sean Taylor and Francisco Bizouarn were crowned the kings of MIQE. Today we’d like to bring you another classic from his majesty Frank. In the slideshow below, you will learn the basics of High Resolution Melt Analysis (HRM), applications, important considerations, assay design and optimization and analysis software. Enjoy. And all hail the king!
Posts Tagged ‘SNP Analysis’
In honor of this launch, we invite you to review some of the resources (including technical notes, review articles and video tutorials) that we have posted on high resolution melt analysis. Feel free to to click on any of the links below for further details:
- High Resolution Melt Analysis Applicatons (publication)
- The Versatility of High-Resolution Melt Analysis (2 resources: review article and technical note)
- Educational Webinar: High-Resolution Melt Analysis (archived version available, registration required)
- A Practical Guide to High Resolution Melt Analysis Genotyping (technical note)
- A Video Tutorial for High Resolution Melt Analysis
- PCR Assay for Chromatin Accessibility (epigenetics methods article)
There is a huge buzz in the molecular biology community around epigenetic factors such DNA methylation and chromosomal orientation and their affect on molecular pathway activity. In two recent posts, we highlighted a new tool for epigenetic analysis which was recognized as one of the most innovative new products of 2011. However, according to a recent study published in Nature Biotechnology, structural variations involving large scale changes in DNA sequences should be just as “buzz-worthy” as epigenetics.
In a review of this article, Wired Science wrote that structural variations in DNA are more specific to individuals than single nucleotide polymorphisms, and may be more responsible than SNPs for genetic difference among people.
Truth be told, epigenetic factors may play a big role in determining which DNA sequences will be modified by our molecular machinery, so perhaps epigenetics does in fact trump (or perhaps even define), structural makeup. One commentator notes that the wired article has nothing to do with epigenetics, however, based on what I’ve noted above, I’m not sure that he is correct.
What are your thoughts? What’s more buzz-worthy? Epigenetics or Genome Structure?
To read more visit Your Genome Structure, Not Genetic Mutations, Makes You Different
In this slideshow, you will learn the latest epigenetic techniques including:
- discriminating epigenetically inactive chromatin from active chromatin
- discriminating between aberrant and Monoallelic DNA methylation
- predicting gene expression levels via chromatin structure assay
- analyzing how DNA methylation affects promoter activity
We have written many posts about the MIQE real time PCR standards that are basic requirements for anyone engaged in real time PCR experimets. Now there is a new tool for all ipod/iphone users to add to their arsenal. A MIQE qPCR app!
The MIQE app helps scientitst review scientific work and check their own project’s MIQE compliance. Plus, the app includes a list of the most current qPCR news and events and “emergency” contact numbers that you can call/email should you have any questions about your qPCR experiments.