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Archive for the ‘Bio-Rad Product Highlight’ Category

Advanced Topics in Cell Sorting

 :: Posted by American Biotechnologist on 10-09-2014

In this webinar, Dan Fox of Propel Labs explores the principles of cell sorting including the theory of droplet cell sorting, how to assess cell sorter performance, and how sorting speed influences results. Dan also discusses the S3™ Cell Sorter’s unique sorting modes, the sort mode process and logic, and how the S3 Sorter simplifies the science of cell sorting.

Fluorescent Imaging Made Easy, Fast, and Intuitive

 :: Posted by American Biotechnologist on 10-02-2014

Bio-Rad Laboratories announces the launch of the ZOE™ Fluorescent Cell Imager, one of the first cell imaging systems able to deliver the power of microscopy in a system that is as easy to use as a tablet. The system includes three fluorescent channels and brightfield to simplify fluorescence imaging for cell culture applications.

Fluorescence microscopes are used in traditional cell culture workflows to confirm transfection efficiency and visualize fluorescent proteins. Performing this task requires training and the process can be time-consuming. Fluorescence microscopes can take 15 minutes to warm up and the systems are often located in a separate facility that is outfitted with a darkroom.

However, the ZOE Fluorescent Cell Imager’s LEDs are instantly ready to use after the power is turned on. Researchers simply place their culture dish onto ZOE’s stage and then access all controls using the LCD touch screen, tapping the screen to capture images and pinching with their fingers to zoom up to 20x magnification. Outfitted with a light shield, ZOE enables users to perform fluorescence experiments in ambient light on their lab benchtop, eliminating the need for a dedicated darkroom.

“Users report they turn on ZOE and know immediately what to do, even those who are imaging cell fluorescence for the first time,” said Veronika Kortisova-Descamps, Bio-Rad product manager, Cell Biology.

Additional Benefits of ZOE

  • Multichannel fluorescence — detection of fluorescence emission at blue, green, and red wavelengths is accomplished with three fluorescence channels optimized for commonly used fluorophores
  • Large field of view — motorized stage and large field of view (0.70 mm2) enable researchers to see more of their sample, faster
  • Manipulate and store images on hard drive — users can merge images for co-expression experiments and store up to 12 GB of image files for export to a USB flash drive

Please visit www.bio-rad.com/ZOEpr for more information about Bio-Rad’s new ZOE Fluorescent Cell Imager.

Better Than Film

 :: Posted by American Biotechnologist on 09-23-2014

Bio-Rad Laboratories announced the launch of its ChemiDoc Touch Imaging System. This system is a significant advance in chemiluminescent western blot detection, surpassing the performance of film and the convenience of other digital imaging systems.

Until now, digital imaging systems failed to deliver the sensitivity and resolution of film. The new ChemiDoc Touch System, however, allows detection of faint bands missed by film and produces publication-quality images.

The ChemiDoc Touch Imaging System is better able than film to detect faint bands.

The ChemiDoc Touch Imaging System is better able than film to detect faint bands.

The ChemiDoc Touch System outperforms film in other ways as well. When using film to image abundant proteins, strong bands quickly saturate and become unquantifiable. Saturated bands can also obscure the signal from adjacent faint bands, making western blotting with film challenging. The ChemiDoc Touch System addresses these issues through its wide dynamic range, which permits easy and reliable quantitation even of highly abundant proteins, and through its ability to optimize exposure for each protein of interest.

Dr. Ernesto Diaz-Flores, PhD, an assistant adjunct professor at the University of California, San Francisco (UCSF), conducted early tests on the Bio-Rad product. Dr. Diaz-Flores used the imager to simultaneously measure protein expression level changes of up to 30 different proteins in samples from leukemia patients to understand how gene mutations alter protein pathways that might represent novel therapeutic targets.

“My goal in collaborating on this project with Bio-Rad was to help develop the next generation technology required to advance protein quantification analysis and its impact in research,” said Dr. Diaz-Flores. “We found that the technology outperformed film and other imaging technologies, as it allows us to simultaneously visualize and quantitate both high and low expression proteins in a matter of seconds. It also allowed us to determine fold induction or protein reduction in high resolution and correlate these levels to drug response in multiple protein assays in a time-efficient manner.”

Capturing images with the ChemiDoc Touch System is easy. Unlike the often sluggish response of other imagers, the ChemiDoc Touch System offers a smooth, intuitive user experience that makes capturing, reviewing, selecting, and exporting images efficient and straightforward.

The imager also allows stain-free imaging, a technology exclusive to Bio-Rad. Using the stain-free enabled V3 Western Workflow™, researchers can quickly determine whether their western blot is proceeding as planned using the imager at multiple built-in checkpoints. Researchers can also use stain-free technology to perform total protein normalization for easier and more reliable protein quantitation.

To learn more about the ChemiDoc Touch System and how it provides a better user experience than film, visit www.bio-rad.com/ChemiDocTouchPR.

Droplet Digital™ PCR Tips & Tricks: ddPCR™ Assay Design

 :: Posted by American Biotechnologist on 09-16-2014

RT-PCR Directly from Cultured Cells

 :: Posted by American Biotechnologist on 09-08-2014

Bio-Rad Laboratories announced the launch of new rapid cell lysis kits that allow researchers to obtain reverse transcription quantitative PCR (RT-qPCR) data directly from cultured cells without the need for a separate RNA purification step. Bio-Rad’s SingleShot™ family of cell lysis RT-qPCR kits provide high-quality gene expression results in less than two hours.

Available column isolation methods for purifying RNA are time-consuming and laborious while other methods that enable RT-qPCR directly from cell lysates can damage the RNA and result in poor genomic DNA clearance. However, Bio-Rad’s SingleShot Kits eliminate those challenges and offer superior reproducibility and accuracy of gene expression results. In addition, minimal setup and pipetting steps create an automation-friendly workflow and, unlike other similar methods, Bio-Rad’s kits do not require an additional pipetting step to stop the cell lysis reaction.

 

The SingleShot Kit removed genomic DNA (Cq > 40) while another available kit resulted in poor genomic DNA clearance.

The SingleShot Kit removed genomic DNA (Cq > 40) while another available kit resulted in poor genomic DNA clearance.

 

“The SingleShot Kits are ideally suited for high-throughput laboratories with large-volume workloads and for researchers who are faced with a limited number of cells and require extreme accuracy in each analysis,” said Paul Streng, senior product manager in the Gene Expression Division of Bio-Rad’s Life Science Group.

Bio-Rad’s SingleShot Kits are the only available kits that include an RNA control template and qPCR assay to help researchers determine optimal cell number and lysate inputs for their RT-qPCR reactions.

SingleShot Kits are validated for use with a wide variety of adherent and suspension cell lines and are offered in multiple formats, including one-step RT-qPCR and two-step RT-qPCR kits that are compatible with either SYBR® Green or probe-based assays. SingleShot Kits are available as a stand-alone cell lysis kit. All SingleShot Kits are validated for use with PrimePCR™ Assays and Panels.