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Archive for the ‘Bio-Rad Product Highlight’ Category

30,000 Scientists Can’t be Wrong

 :: Posted by avi_wener on 05-15-2012

The results are in from SelectScience’s Scientists’ Choice Award and Bio-Rad LaboratoriesTrans-Blot® Turbo™ has taken the prize for Best Life Science Product of 2011. Over 30,000 SelectScience members were invited to vote for their favorite product of 2011 and the Trans-Blot Turbo was given the recognition that it deserves.

Click on the picture below to watch the award ceremony.

To learn more visit www.bio-rad.com/ad/tbt.

More Quantitative Than qPCR

 :: Posted by avi_wener on 05-09-2012

The introduction of real time PCR (qPCR) revolutionized the world of molecular biology and provided scientists with a tool for obtaining truly quantitative nucleic acid data. While qPCR remains one of the most robust tools available on the bench, its resolution power is limited to changes in DNA concentration that are higher than 50%. This limitation is mainly due to the compounding of errors derived from each step in the quantification process. That’s where Droplet Digital PCR enters the picture.

Droplet Digital PCR alleviates the compounding error effect by partitioning samples into 20,000 droplets such that each copy of DNA is encapsulated in its own bubble. Subsequent amplification reactions are then carried out individually which leads to reduce background noise and therefore a more sensitive measurement of low concentrations of nucleic acid that may not have been detectable using qPCR.

Bio-Rad Laboratories‘ Frank Bizouarn recently published an outstanding article in Genetic Engineering and Biotechnology News detailing the benefits of ddPCR including a real life example. The article covers how ddPCR works and its use in gene-expression analysis, copy number variation determination and rare event detection.

Check out Digital PCR: Improving Nucleic Acid Quantification.

Counting cells like the pros

 :: Posted by avi_wener on 04-25-2012

Using Bio-Rad’s TC10 automated cell counter is a breeze! Watch this video and learn how to use the TC10 counter to count cells in less than 30 seconds before splitting them or running an experiment. He discusses the innovative auto-focus technology and how it helps to increase accuracy and reproducibility.

Also see It’s how you count your cells that matter.

Cut Western Blot Time in Half with V3

 :: Posted by avi_wener on 04-16-2012

How anxious are you to get experimental results? Don’t you wish that you could cut those 2 day western blots in half? If so, the V3 workflow method by Bio-Rad Laboratories will surely get you excited.

V3, which stands for visualizing protein separation, verifing protein transfer, and confidently validating blot data via total protein normalization, uses a 5-step process to cut traditional western blotting times down from 2 days to one. The steps include:

  1. 15 minute protein separation
  2. stain free band imaging
  3. three minute protein transfer
  4. instant visualization of protein transfer onto membrane
  5. stain-free total protein normalization for faster quantitative results

For more information visit www.bio-rad.com/ad/V3pr.

Great video explanation of droplet digital PCR

 :: Posted by avi_wener on 03-21-2012

The QX100 Droplet Digital PCR system from Bio-Rad Laboratories is the third generation of PCR technology. The system provides an absolute measure of target DNA molecules with unrivaled performance in precision, accuracy, and sensitivity for quantitative PCR applications. Watch the video below to learn more about this powerful technology.

Related posts:
Introducing the 3rd Generation of PCR
Video Introduction to Droplet Digital PCR
An Advanced Look at Droplet Digital PCR