Archive for the ‘Bio-Rad Product Highlight’ Category
Bio-Rad Laboratories announced the launch of its Automated Droplet Generator (AutoDG™ Instrument) for digital PCR. Combined with the QX200™ Droplet Reader and a laptop computer with QuantaSoft™ Software, the QX200™ AutoDG™ Droplet Digital™ PCR System provides a worry-free, automated way to generate droplets for high-quality data.
“With a quick setup, you’re able to walk away and return to a full plate of droplets ready for thermal cycling and analysis,” said Carolyn Reifsnyder, Bio-Rad marketing manager, Digital Biology Center.
Generating droplets with a hand pipet can be labor intensive and time consuming, and variations in pipetting habits among different users can cause some degree of inconsistency in the resulting data. Bio-Rad’s AutoDG Instrument creates droplets quickly, reproducibly, and reliably. At maximum capacity, the system can generate droplets for 96 wells in less than 45 minutes.
“Droplet Digital PCR is sensitive enough to detect differences in people’s pipetting habits,” said Viresh Patel, Bio-Rad senior marketing manager, Digital Biology Center. “This instrument eliminates that variability.”
The AutoDG Instrument has its own hood and HEPA filter, reducing contamination so researchers can create droplets on a standard laboratory bench, without the need for a PCR cabinet or cleanroom. Adding to the instrument’s ease of use is a color touch-screen interface and deck lighting that guides the operator through system setup to ensure no steps are forgotten. Confirmation is provided once the required consumables are loaded correctly and 45 minutes later the droplets are ready.
Bio-Rad’s AutoDG Instrument can generate droplets for fluorescent probe–based detection or EvaGreen dye–based detection. The instrument is compatible with the QX100™ and QX200 Droplet Digital PCR Systems.
For more information on Bio-Rad’s AutoDG Droplet Digital PCR System, please visit www.bio-rad.com/AutoDGpr.
Bio-Rad Laboratories announced the launch of new PrimePCR Assays for Droplet Digital PCR (ddPCR™). This release expands Bio-Rad’s current offering of predesigned and fully wet-lab validated assays by an additional 100 assays for copy number variation (CNV) and 92 assays for mutation detection. Bio-Rad will also be adding 5,620 mutation detection assays that were created using the same algorithms and design rules as the wet-lab validated assays.
Bio-Rad’s ddPCR technology provides an absolute measure of target DNA molecules without the need for a standard curve. Together with PrimePCR Mutation Detection Assays, this technology enables detection of one mutant molecule in a background of 2,000 wild-type molecules. Measuring these extremely low levels of mutation abundance could lead to the development of new, less invasive and more sensitive diagnostics. In addition, the high precision and absolute quantification made possible by PrimePCR ddPCR Assays enable the quantitative discrimination required to resolve small fold changes in gene copy numbers for CNV analysis.
Highly studied gene targets
Among the genes that can now be targeted using PrimePCR ddPCR Assays is paired box gene 2 (PAX2), a CNV gene target that is suspected to have both oncogenic and tumor suppression functions in ovarian cancers. Another notable CNV assay released as part of this launch detects the neurexin-1-alpha gene (NRXN1), which has been linked to a range of neurodevelopmental disorders, including autism spectrum disorders, schizophrenia, and intellectual disability.
The new mutation detection PrimePCR ddPCR Assays include those for fms-related tyrosine kinase (FLT) mutations that are observed in acute myeloid leukemia (AML) patients. Mutations in the fms-like tyrosine kinase 3 (FLT3) gene represent one of the most frequently encountered and clinically challenging classes of AML mutations. This release also includes several assays that detect mutations in the neuroblastoma RAS (NRAS) gene, including the G12V mutation that has recently been linked to poor prognosis in malignant melanoma.
PrimePCR ddPCR Assays are compatible with all Bio-Rad Droplet Digital PCR Systems, including the QX200™ AutoDG™ Droplet Digital™ PCR System.
For more information on Bio-Rad’s PrimePCR products, please visit www.bio-rad.com/PrimePCRpr.
In this webinar, Dan Fox of Propel Labs explores the principles of cell sorting including the theory of droplet cell sorting, how to assess cell sorter performance, and how sorting speed influences results. Dan also discusses the S3™ Cell Sorter’s unique sorting modes, the sort mode process and logic, and how the S3 Sorter simplifies the science of cell sorting.
Bio-Rad Laboratories announces the launch of the ZOE™ Fluorescent Cell Imager, one of the first cell imaging systems able to deliver the power of microscopy in a system that is as easy to use as a tablet. The system includes three fluorescent channels and brightfield to simplify fluorescence imaging for cell culture applications.
Fluorescence microscopes are used in traditional cell culture workflows to confirm transfection efficiency and visualize fluorescent proteins. Performing this task requires training and the process can be time-consuming. Fluorescence microscopes can take 15 minutes to warm up and the systems are often located in a separate facility that is outfitted with a darkroom.
However, the ZOE Fluorescent Cell Imager’s LEDs are instantly ready to use after the power is turned on. Researchers simply place their culture dish onto ZOE’s stage and then access all controls using the LCD touch screen, tapping the screen to capture images and pinching with their fingers to zoom up to 20x magnification. Outfitted with a light shield, ZOE enables users to perform fluorescence experiments in ambient light on their lab benchtop, eliminating the need for a dedicated darkroom.
“Users report they turn on ZOE and know immediately what to do, even those who are imaging cell fluorescence for the first time,” said Veronika Kortisova-Descamps, Bio-Rad product manager, Cell Biology.
Additional Benefits of ZOE
- Multichannel fluorescence — detection of fluorescence emission at blue, green, and red wavelengths is accomplished with three fluorescence channels optimized for commonly used fluorophores
- Large field of view — motorized stage and large field of view (0.70 mm2) enable researchers to see more of their sample, faster
- Manipulate and store images on hard drive — users can merge images for co-expression experiments and store up to 12 GB of image files for export to a USB flash drive
Please visit www.bio-rad.com/ZOEpr for more information about Bio-Rad’s new ZOE Fluorescent Cell Imager.