Designing good qPCR assays can be fun! Learn how to overcome difficult assays, designs and optimization while conforming to the MIQE guidelines.
Posts Tagged ‘high-resolution melt analysis’
In honor of this launch, we invite you to review some of the resources (including technical notes, review articles and video tutorials) that we have posted on high resolution melt analysis. Feel free to to click on any of the links below for further details:
- High Resolution Melt Analysis Applicatons (publication)
- The Versatility of High-Resolution Melt Analysis (2 resources: review article and technical note)
- Educational Webinar: High-Resolution Melt Analysis (archived version available, registration required)
- A Practical Guide to High Resolution Melt Analysis Genotyping (technical note)
- A Video Tutorial for High Resolution Melt Analysis
- PCR Assay for Chromatin Accessibility (epigenetics methods article)
We have written many posts about the MIQE real time PCR standards that are basic requirements for anyone engaged in real time PCR experimets. Now there is a new tool for all ipod/iphone users to add to their arsenal. A MIQE qPCR app!
The MIQE app helps scientitst review scientific work and check their own project’s MIQE compliance. Plus, the app includes a list of the most current qPCR news and events and “emergency” contact numbers that you can call/email should you have any questions about your qPCR experiments.
An easy-to-use qPCR resource, Bio-Rad’s Real-Time PCR iPhone application includes the Real-Time PCR Applications Guide for researchers who want to learn more about designing, analyzing, and optimizing real-time PCR experiments. Another feature is the qPCR Doctor, an interactive troubleshooting tool for resolving problems relating to real-time PCR assays. The Real-Time PCR iPhone Application also includes a qPCR Assay Design section which provides guidance for designing a qPCR assay, information on validating and optimizing your qPCR assay, and different methods for analyzing qPCR data. This application puts three of Bio-Rad’s best real-time PCR resources at your fingertips.
High resolution melt (HRM) analysis is a relatively new technique used in detecting small variations in DNA sequences between varying populations. Important applications of HRM include SNP analysis, genotyping and methylation analysis.
In the following 20 minute tutorial presented by Sean Taylor, Field Application Specialist, Bio-Rad Laboratories, you will learn the basics of high resolution melt analysis and how to practically use it in your research. The video contains information on:
- what is high resolution melt analysis, how does it work and why it is useful
- various applications for high resolution melt analysis including single nucleotide polymorphism (SNP) analysis and DNA methylation analysis
- the difference between a standard melt curved performed in routine QPCR and HRM
- the difference between non-saturation dyes such as SYBR green and saturation dyes such as evagreen
- the definition of the four class types of SNPS
- critical factors for good experimental design
- what technique you should be using for your SNP analysis (it is not one size fits all…think HRM VS Sequenome VS multiplex array
- and more!!!
For more information on HRM see A Practical Guide to High Resolution Melt Analysis Genotyping