Posts Tagged ‘Bio-Rad Laboratories’

Total Protein Normalization on Western Blots

 :: Posted by American Biotechnologist on 10-23-2014

Easy Detection of Low Level Mutations

 :: Posted by American Biotechnologist on 10-20-2014

Bio-Rad Laboratories announced the launch of new PrimePCR Assays for Droplet Digital PCR (ddPCR™). This release expands Bio-Rad’s current offering of predesigned and fully wet-lab validated assays by an additional 100 assays for copy number variation (CNV) and 92 assays for mutation detection. Bio-Rad will also be adding 5,620 mutation detection assays that were created using the same algorithms and design rules as the wet-lab validated assays.

Bio-Rad’s ddPCR technology provides an absolute measure of target DNA molecules without the need for a standard curve. Together with PrimePCR Mutation Detection Assays, this technology enables detection of one mutant molecule in a background of 2,000 wild-type molecules. Measuring these extremely low levels of mutation abundance could lead to the development of new, less invasive and more sensitive diagnostics. In addition, the high precision and absolute quantification made possible by PrimePCR ddPCR Assays enable the quantitative discrimination required to resolve small fold changes in gene copy numbers for CNV analysis.

Highly studied gene targets
Among the genes that can now be targeted using PrimePCR ddPCR Assays is paired box gene 2 (PAX2), a CNV gene target that is suspected to have both oncogenic and tumor suppression functions in ovarian cancers. Another notable CNV assay released as part of this launch detects the neurexin-1-alpha gene (NRXN1), which has been linked to a range of neurodevelopmental disorders, including autism spectrum disorders, schizophrenia, and intellectual disability.

The new mutation detection PrimePCR ddPCR Assays include those for fms-related tyrosine kinase (FLT) mutations that are observed in acute myeloid leukemia (AML) patients. Mutations in the fms-like tyrosine kinase 3 (FLT3) gene represent one of the most frequently encountered and clinically challenging classes of AML mutations. This release also includes several assays that detect mutations in the neuroblastoma RAS (NRAS) gene, including the G12V mutation that has recently been linked to poor prognosis in malignant melanoma.

PrimePCR ddPCR Assays are compatible with all Bio-Rad Droplet Digital PCR Systems, including the QX200™ AutoDG™ Droplet Digital™ PCR System.

For more information on Bio-Rad’s PrimePCR products, please visit www.bio-rad.com/PrimePCRpr.

Advanced Topics in Cell Sorting

 :: Posted by American Biotechnologist on 10-09-2014

In this webinar, Dan Fox of Propel Labs explores the principles of cell sorting including the theory of droplet cell sorting, how to assess cell sorter performance, and how sorting speed influences results. Dan also discusses the S3™ Cell Sorter’s unique sorting modes, the sort mode process and logic, and how the S3 Sorter simplifies the science of cell sorting.

Image Lab™ Software: Learn Volume Analysis from the Experts

 :: Posted by American Biotechnologist on 09-29-2014

Presented by: Ben Wang, PhD Senior Technical Support Specialist

Presented by:
Ben Wang, PhD
Senior Technical Support Specialist

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Join us for a 30 minute live webinar developed and delivered by our knowledgeable Technical Support Team.
TomorrowTuesday, September 30, 2014 | 10:00 AM Pacific
As you get ready to use your new system, we will provide you with an opportunity to learn about the analysis tools built into the Image Lab software. This training will cover the steps to use our volume tools to successfully quantitate bands, dot blots, and arrays.

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Better Than Film

 :: Posted by American Biotechnologist on 09-23-2014

Bio-Rad Laboratories announced the launch of its ChemiDoc Touch Imaging System. This system is a significant advance in chemiluminescent western blot detection, surpassing the performance of film and the convenience of other digital imaging systems.

Until now, digital imaging systems failed to deliver the sensitivity and resolution of film. The new ChemiDoc Touch System, however, allows detection of faint bands missed by film and produces publication-quality images.

The ChemiDoc Touch Imaging System is better able than film to detect faint bands.

The ChemiDoc Touch Imaging System is better able than film to detect faint bands.

The ChemiDoc Touch System outperforms film in other ways as well. When using film to image abundant proteins, strong bands quickly saturate and become unquantifiable. Saturated bands can also obscure the signal from adjacent faint bands, making western blotting with film challenging. The ChemiDoc Touch System addresses these issues through its wide dynamic range, which permits easy and reliable quantitation even of highly abundant proteins, and through its ability to optimize exposure for each protein of interest.

Dr. Ernesto Diaz-Flores, PhD, an assistant adjunct professor at the University of California, San Francisco (UCSF), conducted early tests on the Bio-Rad product. Dr. Diaz-Flores used the imager to simultaneously measure protein expression level changes of up to 30 different proteins in samples from leukemia patients to understand how gene mutations alter protein pathways that might represent novel therapeutic targets.

“My goal in collaborating on this project with Bio-Rad was to help develop the next generation technology required to advance protein quantification analysis and its impact in research,” said Dr. Diaz-Flores. “We found that the technology outperformed film and other imaging technologies, as it allows us to simultaneously visualize and quantitate both high and low expression proteins in a matter of seconds. It also allowed us to determine fold induction or protein reduction in high resolution and correlate these levels to drug response in multiple protein assays in a time-efficient manner.”

Capturing images with the ChemiDoc Touch System is easy. Unlike the often sluggish response of other imagers, the ChemiDoc Touch System offers a smooth, intuitive user experience that makes capturing, reviewing, selecting, and exporting images efficient and straightforward.

The imager also allows stain-free imaging, a technology exclusive to Bio-Rad. Using the stain-free enabled V3 Western Workflow™, researchers can quickly determine whether their western blot is proceeding as planned using the imager at multiple built-in checkpoints. Researchers can also use stain-free technology to perform total protein normalization for easier and more reliable protein quantitation.

To learn more about the ChemiDoc Touch System and how it provides a better user experience than film, visit www.bio-rad.com/ChemiDocTouchPR.