Posts Tagged ‘Bio-Rad Laboratories’

Droplet Digital™ PCR Tips & Tricks: ddPCR™ Assay Design

 :: Posted by American Biotechnologist on 09-16-2014

RT-PCR Directly from Cultured Cells

 :: Posted by American Biotechnologist on 09-08-2014

Bio-Rad Laboratories announced the launch of new rapid cell lysis kits that allow researchers to obtain reverse transcription quantitative PCR (RT-qPCR) data directly from cultured cells without the need for a separate RNA purification step. Bio-Rad’s SingleShot™ family of cell lysis RT-qPCR kits provide high-quality gene expression results in less than two hours.

Available column isolation methods for purifying RNA are time-consuming and laborious while other methods that enable RT-qPCR directly from cell lysates can damage the RNA and result in poor genomic DNA clearance. However, Bio-Rad’s SingleShot Kits eliminate those challenges and offer superior reproducibility and accuracy of gene expression results. In addition, minimal setup and pipetting steps create an automation-friendly workflow and, unlike other similar methods, Bio-Rad’s kits do not require an additional pipetting step to stop the cell lysis reaction.

 

The SingleShot Kit removed genomic DNA (Cq > 40) while another available kit resulted in poor genomic DNA clearance.

The SingleShot Kit removed genomic DNA (Cq > 40) while another available kit resulted in poor genomic DNA clearance.

 

“The SingleShot Kits are ideally suited for high-throughput laboratories with large-volume workloads and for researchers who are faced with a limited number of cells and require extreme accuracy in each analysis,” said Paul Streng, senior product manager in the Gene Expression Division of Bio-Rad’s Life Science Group.

Bio-Rad’s SingleShot Kits are the only available kits that include an RNA control template and qPCR assay to help researchers determine optimal cell number and lysate inputs for their RT-qPCR reactions.

SingleShot Kits are validated for use with a wide variety of adherent and suspension cell lines and are offered in multiple formats, including one-step RT-qPCR and two-step RT-qPCR kits that are compatible with either SYBR® Green or probe-based assays. SingleShot Kits are available as a stand-alone cell lysis kit. All SingleShot Kits are validated for use with PrimePCR™ Assays and Panels.

Developing a Cell Sorter: An Insider’s Perspective

 :: Posted by American Biotechnologist on 08-28-2014

High-Performance Immunoprecipitation at an Affordable Price

 :: Posted by American Biotechnologist on 08-12-2014

Bio-Rad Laboratories, Inc. announced that it has launched its SureBeads Magnetic Bead System, which provides researchers with a faster and easier alternative to agarose beads for immunoprecipitation for as low as half the price of other available magnetic bead systems. Benefits include reduced antibody consumption and sample loss, very low nonspecific binding, and optimized IgG binding capacity.

Researchers have traditionally used agarose beads to precipitate and enrich proteins prior to western blotting or mass spectrometry. This process is time-consuming and labor-intensive, and often causes the beads to perform poorly. Since agarose beads are porous, the antibody can remain trapped inside the bead and therefore is unable to properly bind the protein of interest, requiring researchers to use more antibody.

Although magnetic beads are often more expensive, they are an attractive alternative to agarose beads for several reasons, including ease of use at the bench: magnetization is faster and more convenient than centrifugation for sample precipitation and washing steps. With the launch of the SureBeads System, scientists now have access to a high-performance, cost-effective magnetic bead system.

SureBeads Protein A and G Conjugated Magnetic Beads are designed to work with the proprietary SureBeads 16-Tube Magnetic Rack, which offers a one-piece removable magnetic strip to improve sample handling and eliminate repeated centrifugation steps, so researchers are able to achieve results up to six times faster.

“I have used magnetic bead–based systems for more than three years now, and the quality of the new SureBeads System is among the best in the field,” said Oliver Wueseke, PhD student at the Max Planck Institute for Molecular Cell Biology and Genetics in Dresden, Germany. “The real innovation resides in the rack. The slidable magnet, preferred fixation of tubes in the rack, and spacing between tubes that allows easy access to the tube lids make the SureBeads System easy to handle, faster, and more convenient.“

Visit www.bio-rad.com/NewSureBeads for more information about the SureBeads System and to learn about current product promotions.

Introduction to Droplet Digital PCR

 :: Posted by American Biotechnologist on 07-31-2014