Posts Tagged ‘Bio-Rad Laboratories’
Dr. Christopher Fraser and the members of his lab in the Department of Molecular and Cellular Biology at the University of California, Davis, want to better understand the fundamental cellular process of protein synthesis. The genomic DNA in each cell of an organism encodes the same set of genes, but gene expression is regulated so that only certain proteins are produced at appropriate times in each cell, resulting in diverse cellular functions. The control of the first step of protein synthesis is the regulation of DNA transcription into mRNA. The next step, the translation of mRNA into protein by the ribosome, is also tightly controlled in normal cells, and dysfunctional regulation is responsible for disorders such as autism, Type I diabetes, and cancer.
Bio-Rad Launches New ddPCR Library Quantification Kit to Optimize Performance of Illumina NGS Platforms:: Posted by American Biotechnologist on 04-17-2013
Bio-Rad Laboratories, Inc., today announced the availability of its new ddPCR library quantification kit for Illumina TruSeq sample preparation protocols. Used with Bio-Rad’s QX100™ Droplet Digital™ PCR system, the new kit offers researchers a way to precisely and directly measure amplifiable library concentrations.
The TruSeq sample preparation method is the technique behind Illumina’s MiSeq and HiSeq next-generation sequencing (NGS)platforms. Using the ddPCR library quantification kit to quantify TruSeq DNA libraries maximizes the number of useable reads, enables consistent loading, and optimizes the utilization of every sequencing run. The resulting data provide additional measures of library quality not provided by other methods, including the percentage of nonamplifiable species such as adaptor dimers as well as the size range of library inserts.
Additional key benefits of the ddPCR library quantification kit include:
- Superior performance — reduces PCR bias due to sample partitioning during quantification
- Simple workflow — easily incorporated into the TruSeq library construction workflow
- Efficient utilization of sequencing runs — provides fluorescence amplitude data, a metric for library quality
Kits for other NGS platforms are also in development. For more information on the ddPCR library quantification kit, please visit http://bit.ly/ddPCR_QKL.