Posts Tagged ‘qPCR’

Overcoming PCR Inhibitors

 :: Posted by American Biotechnologist on 07-15-2014

Sso advanced universal inhibitorBio-Rad Laboratories announced the launch of the SsoAdvanced™ Universal Inhibitor-Tolerant SYBR® Green Supermix, a proven solution for obtaining reproducible, optimal-quality qPCR data from the most challenging samples.

Generating quality qPCR data from difficult-to-amplify samples such as crude lysates from plants, tissues, formalin-fixed paraffin-embedded samples, and other less-than-ideal sources that contain PCR inhibitors can be challenging and frustrating. In these situations, PCR may be greatly inhibited, making it nearly impossible for researchers to obtain reliable data, wasting time and money.

The formulation of the SsoAdvanced™ Universal Inhibitor-Tolerant SYBR® Green Supermix has been optimized and validated to tolerate a wider spectrum of PCR inhibitors than competing SYBR® Green reagents. These inhibitors include those found in crude lysates, polysaccharides and polyphenols, and various reagents left over from sample prep such as ethanol, isopropanol, EDTA, and sodium chloride see performance data for all inhibitors.

As with all SsoAdvanced Supermixes, the inhibitor-tolerant version includes Bio-Rad’s Sso7d-fusion polymerase, which is engineered for enhanced qPCR performance. The supermix is compatible with all real-time PCR instruments and functions under any reaction condition.

Visit www.bio-rad.com/SUIT1 to learn more about SsoAdvanced™ Universal Inhibitor-Tolerant SYBR® Green Supermix.

Improving Visibility of qPCR Reaction Mixtures

 :: Posted by American Biotechnologist on 05-14-2014

Bio-Rad Laboratories recently announced the launch of its Precision Blue real-time PCR dye, making it easier for researchers to set up real-time PCR (qPCR) experiments.

The blue dye improves the visibility of qPCR reaction mixtures, enabling loading of small volume reactions with greater speed, accuracy, and precision. Applications such as single nucleotide polymorphism (SNP) genotyping and high-throughput gene expression analysis using 384-well plates will particularly benefit from use of this dye.

Precision Blue dye is compatible with Bio-Rad’s universal qPCR supermixes (iTaq™ universal and SsoAdvanced™ universal supermixes) and does not interfere with SYBR® Green- or probe-based detection. The dye can be used with all Bio-Rad CFX real-time PCR systems and all other vendors’ real-time PCR systems.

For convenience, Precision Blue can be added directly to the supermix or used as a component of a reaction master mix. The dye is concentrated 200x, and delivers 500 x 20 µl reactions.

For more information about Precision Blue dye, please visit www.bio-rad.com/PrecisionBlue.

Bio-Rad Sponsors New Version of MIQE qPCR App for Real-Time PCR and Digital PCR

 :: Posted by American Biotechnologist on 08-28-2013

miqe appBio-Rad Laboratories is sponsoring a new version of the MIQE qPCR app. Researchers can use the new version to validate their digital PCR (dPCR) experiments according to the recently published digital MIQE (dMIQE) guidelines (Huggett et al. 2013).

Professors Michael Pfaffl (TU Munich, Weihenstephan, Germany) and Afif Nour (King Abdulaziz University, Saudi Arabia) designed the original MIQE qPCR app to help researchers improve their real-time PCR (qPCR) assay protocols by making it easier to adopt a set of best practices described in an earlier publication: The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments (Bustin et al. 2009). Progress bars in the app show the percentage of assay compliance as each item in the MIQE checklist is completed.

“We were pleased to find that the MIQE qPCR app encouraged our customers to follow the new MIQE guidelines,” said Jean-Pierre Dakkak, a laboratory equipment trading company manager.

“Researchers are really eager to learn how this app can make their life easier,” said Dr. Nour. “They report it instills confidence in the validity of every qPCR or dPCR project.”

The new updates include:

  • Project-specific checklists — checklists remove unnecessary items; for example, reverse transcription items are irrelevant in DNA research
  • Updated references — researchers can stay current with the latest MIQE and dMIQE literature and qPCR symposiums
  • Easy export — users can save their projects and share them with colleagues

The MIQE qPCR app runs on the Apple iPhone, iPod touch, and iPad. It has been downloaded more than 6,500 times in 87 countries. To get your copy, visit http://bit.ly/MIQEapp.

Innovative qPCR Reagents for Superior Performance

 :: Posted by American Biotechnologist on 04-29-2013

Bio-Rad Receives Award in Quantitative and Digital PCR Instrumentation

 :: Posted by American Biotechnologist on 11-19-2012

Bio-Rad Laboratories, Inc. announced it has received the Frost & Sullivan 2012 North American Market Penetration Leadership Award in Quantitative and Digital PCR Instrumentation. This annual award honors the company that has demonstrated excellence by growing market share at the fastest rate in its industry, as measured by revenues or units sold.

Frost & Sullivan, a global growth consulting company, cited findings from its best practices research that show Bio-Rad has built a solid reputation in amplification technologies. According to Frost & Sullivan’s research, Bio-Rad continually releases innovative products to earn customer loyalty and gain market share, with particularly strong market positions in both the real-time PCR (qPCR) and digital (dPCR) instrumentation markets.

“Bio-Rad clearly exhibits some of the key traits shared by the most successful companies: the ability to locate and solve unmet needs in the markets they serve and a dedication to continuous R&D,” said Christi Bird, life sciences senior industry analyst at Frost & Sullivan. “Bio-Rad’s commitment to innovation, coupled with its keen focus on other qualities valued most by its customers, has resulted in a strong and growing market position that makes the company an ideal recipient of this year’s Frost & Sullivan Market Penetration Leadership Award.”

“We are honored to receive recognition of our leadership in the real-time and digital PCR markets,” said Rachel Scott, amplification instruments business unit marketing manager at Bio-Rad. “The Frost & Sullivan award is a reflection of how we strive to develop innovative new solutions that align with the needs of the research community in terms of both technology and pricing.”

Frost & Sullivan expects Bio-Rad to continue its market share gains in qPCR with several instruments launched in 2011, including the CFX96 Touch™ and CFX Connect™ real-time PCR detection systems.

In the dPCR market, Frost & Sullivan believes the QX100™ Droplet Digital™ PCR system holds the greatest promise of all dPCR instruments currently on the market, due to its simplicity, performance, and affordability. The QX100 system has already received significant attention from pharmaceutical companies and cancer research laboratories, leading Frost & Sullivan to believe that the instrument will be the early market leader in the expanding dPCR market.

“Bio-Rad has also seen a great deal of interest from the growing molecular diagnostic market,” said James Lee, Droplet Digital PCR commercialization marketing manager at Bio-Rad. “We plan to participate in this market in the near future using our ddPCR™ technology.”

Bio-Rad was honored with this recognition at Frost & Sullivan’s 2012 Excellence in Best Practices Awards Banquet on November 8 in San Antonio, Texas. The event highlighted companies, strategies, processes, and executives that have achieved world-class performance within their industries.

For more information about Bio-Rad’s PCR products, visit http://bit.ly/PCR_Products.