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In this video, Dr. Sean Taylor, Field Applications Specialist, Bio-Rad Laboratories, demonstrates how sample quality and reference gene selection effect data analysis and interpretation in real-time quantitative PCR (qPCR) experiments. The presentation is in accordance with the previously published MIQE guidelines.
For enhanced viewing, click on the full-screen mode button on the bottom right hand corner of the video.
The tech note that was written last year entitled: “A practical approach to RT-qPCR- Publishing data that conform to the MIQE guidelines” by Sean Taylor, Michael Wakem, Greg Dijkman, Marwan Alsarraj and Marie Nguyen has been published in Methods (2010 Apr;50(4):S1-5.).
Taylor S, Wakem M, Dijkman G, Alsarraj M, & Nguyen M (2010). A practical approach to RT-qPCR-Publishing data that conform to the MIQE guidelines. Methods (San Diego, Calif.), 50 (4) PMID: 20215014
To support the MIQE Guidelines and best practices for qPCR experiments, Bio-Rad has initiated a half-day qPCR workshop to:
1. Analyze your own RNA samples to assure that they are of appropriate quality and purity for qPCR.
2. Demonstrate the importance of accurate pipetting, good technique and quality reagents with a pipetting competition (prizes will be awarded!).
3. Teach you how to design a solid experimental approach for excellent results that conform to the recently published MIQE guidelines (Bustin et al. 2009; Taylor et al. 2010).
If you are interested in holding a workshop in your area please send an email to avi_wener@bio-rad.com. Be sure to leave your name, email address, phone number and location and someone will get back to you shortly.
