In part I of the 2-D electrophoresis series (2DGE), Bio-Rad Laboratories’ Anton Posch talked about proper sample preparation techniques for good 2DGE. In this second and final part of the series, Dr. Posch covers IEF separation, using immobilized pH gradient (IPG) strips, running second-dimension PAGE, spot detection, gel matching, and data analysis. If you want to perform great 2DGE, it is well worth your while to watch these two tutorials.
Posts Tagged ‘2D Gel Electrophoresis’
Great proteomics tutorial from Bio-Rad Laboratories.
Hercules, CA — March 13, 2012 — Bio-Rad Laboratories, Inc.’s PROTEAN i12 IEF system won a Laboratory Equipment Readers’ Choice Award in the Basic Lab Equipment category. Theaward will be presented today at a special reception during the Pittcon Conference & Expo 2012 being held at the Orange County Convention Center in Orlando, FL.
“The PROTEAN i12 IEF system individually controls each of its 12 lanes, allowing researchers to generate reproducible 2-D gels in less time and with total confidence,” said Renee Lemaire-Adkins, Bio-Rad Marketing Manager, Lab Separations Division. “It is a great honor to have our IEF technology recognized by the researchers and laboratory professionals who compose Laboratory Equipment’s readership.”
The 4th annual award celebrates “excellence in product design and performance for the tools and materials used by scientists and engineers in research laboratories.” Laboratory Equipment readers voted for products online.
The PROTEAN i12 IEF system is the industry’s only isoelectric focusing (IEF) system designed to simultaneously run up to 12 immobilized pH gradient (IPG) strips in 12 independently programmed lanes, which allows users to confidently run 12 different conditions at one time. Although many available IEF systems have multiple lanes, all but the PROTEAN i12 system depend on a single power supply, allowing only one set of conditions to be run at a time.
By independently controlling voltage and current levels in each lane, the PROTEAN i12 IEF cell allows users to optimize different sample and pH conditions and run samples from different experiments simultaneously.The independent lane control prevents lane-to-lane sample interference, resulting in faster, more accurate, reliable, and reproducible focusing.
Visit http://bit.ly/proteani12pr or Bio-Rad’s Pittcon Booth #1412 to learn more about the PROTEAN i12 IEF system. You can find more about the Laboratory Equipment Reader’s Choice Award at http://bit.ly/2012readerschoice.