Bio-Rad Laboratories, Inc. today announced the launch of its Mini-PROTEAN TGX (Tris-glycine extended) Stain-Free precast gels in gradient formats 4–15% and 4–20%. Ideal for samples that contain a broad range of protein sizes, the new formats extend Bio-Rad’s line of mini- and midi-sized stain-free precast gels, providing researchers with the fastest separation and in-gel protein visualization capabilities available for Laemmli-based PAGE.
Typical polyacrylamide gel staining methods that follow Coomassie protocols can take four or more hours to complete. The Mini-PROTEAN TGX Stain-Free precast gels include a unique trihalo compound that allows rapid fluorescent detection of proteins in gels or on membranes without the need for staining. When researchers use the Mini-PROTEAN TGX Stain-Free precast gels as part of Bio-Rad’s V3 Western Workflow™, they can achieve complete protein separation, gel imaging, and data analysis in 20 minutes.
Other key benefits of Mini-PROTEAN TGX Stain-Free precast gels in gradient format include:
Greater transfer efficiency — proteins can be transferred onto PVDF or nitrocellulose membranes in as little as 3 minutes with the Trans-Blot® Turbo™ system
Versatility — the same gel can be used for western blotting, standard staining methods, and mass spectrometry analysis of proteins
Cost-effectiveness — unlike other precast gels that require specialized buffer systems and stains, the Mini-PROTEAN TGX Stain-Free precast gels use inexpensive standard Laemmli buffer and do not require staining and destaining, keeping running costs low
The Mini-PROTEAN TGX Stain-Free precast gels are available in a wide range of percentages: 7.5%, 10%, 12%, 4–15%, 4–20%, and Any kD™ (offers optimal resolution in the 10–100 kD range). Gels can be stored for up to 12 months without loss of performance.
For product details or to order Mini-PROTEAN TGX Stain-Free gels, visit http://bit.ly/MP_SFTGX.