Posts Tagged ‘droplet digital pcr’

Automated Droplet Generator Saves Time and Improves Data Quality

 :: Posted by American Biotechnologist on 11-03-2014

Bio-Rad Laboratories announced the launch of its Automated Droplet Generator (AutoDG™ Instrument) for digital PCR. Combined with the QX200™ Droplet Reader and a laptop computer with QuantaSoft™ Software, the QX200™ AutoDG™ Droplet Digital™ PCR System provides a worry-free, automated way to generate droplets for high-quality data.

“With a quick setup, you’re able to walk away and return to a full plate of droplets ready for thermal cycling and analysis,” said Carolyn Reifsnyder, Bio-Rad marketing manager, Digital Biology Center.

Generating droplets with a hand pipet can be labor intensive and time consuming, and variations in pipetting habits among different users can cause some degree of inconsistency in the resulting data. Bio-Rad’s AutoDG Instrument creates droplets quickly, reproducibly, and reliably. At maximum capacity, the system can generate droplets for 96 wells in less than 45 minutes.

“Droplet Digital PCR is sensitive enough to detect differences in people’s pipetting habits,” said Viresh Patel, Bio-Rad senior marketing manager, Digital Biology Center. “This instrument eliminates that variability.”

The AutoDG Instrument has its own hood and HEPA filter, reducing contamination so researchers can create droplets on a standard laboratory bench, without the need for a PCR cabinet or cleanroom. Adding to the instrument’s ease of use is a color touch-screen interface and deck lighting that guides the operator through system setup to ensure no steps are forgotten. Confirmation is provided once the required consumables are loaded correctly and 45 minutes later the droplets are ready.

Bio-Rad’s AutoDG Instrument can generate droplets for fluorescent probe–based detection or EvaGreen dye–based detection. The instrument is compatible with the QX100™ and QX200 Droplet Digital PCR Systems.

For more information on Bio-Rad’s AutoDG Droplet Digital PCR System, please visit www.bio-rad.com/AutoDGpr.

Droplet Digital™ PCR Tips & Tricks: ddPCR™ Assay Design

 :: Posted by American Biotechnologist on 09-16-2014

Using Droplet Digital™ PCR to Quantify Early Rejection Biomarkers in Transplant Patients

 :: Posted by American Biotechnologist on 08-18-2014

Introduction to Droplet Digital PCR

 :: Posted by American Biotechnologist on 07-31-2014

A Robust Method for Detecting DNA Methylation

 :: Posted by American Biotechnologist on 06-09-2014

DNA methylation is one of the most studied epigenetic modifications. DNA methylation plays an important role in a number of physiological processes as well as common diseases such as cancer and neurodegenerative disorders. Recent data have shown a correlation of DNA methylation and disease status. However, there is an unmet need for a sensitive yet robust method to analyze DNA methylation in clinical samples.

In the bulletin “Droplet Digital PCR: Detection of DNA Methylation”, Bio-Rad Laboratories demonstrates teh unprecedented sensitivity, linearity and robustness of DNA methylation detection by ddPCR in a model system for an imprinted gene involved in neurological disorders.

Read “Droplet Digital PCR: Detection of DNA Methylation” for more information.