Posts Tagged ‘Criterion Stain Free’

TGX Stain-Free™ Precast Gels Facilitate LDL Research on Potential New Drug Targets

 :: Posted by American Biotechnologist on 10-24-2012

Cholesterol is a waxy fat produced in the human body that is associated with both low-density lipoproteins (LDLs) and high-density lipoproteins (HDLs). Due to genetic predisposition and lifestyle factors such as diet and exercise habits, LDL-associated cholesterol (LDL-C), the “bad” form of cholesterol, can accumulate on arterial walls and harden to form a plaque, causing a narrowing and loss of elasticity in these blood vessels. This process, known as atherosclerosis, or hardening of the arteries, increases the risk of cardiovascular diseases like heart attack and stroke.

Dr Frederick Bauzon, a postdoctoral researcher in Dr Ronald Krauss’s lab at the Children’s Hospital Oakland Research Institute (CHORI), is investigating the role of a candidate gene associated with HMGCR transcript levels discovered in a linkage study. The gene expression levels are “reduced” using a small interfering RNA (siRNA) and the effects on expression of the low-density lipoprotein receptor protein (LDLR) are examined. The established mechanism is that reducing LDLR expression decreases the amount of available receptor protein, which normally binds LDLs circulating in the bloodstream and transports them back inside the cell. The pathway was discovered ~20 years ago and has been of interest in drug target development due to its regulating role.

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Bio-Rad Introduces Mini-PROTEAN® TGX Stain-Free™ Precast Gels in Gradient Format

 :: Posted by American Biotechnologist on 09-12-2012

Bio-Rad Laboratories, Inc. today announced the launch of its Mini-PROTEAN TGX (Tris-glycine extended) Stain-Free precast gels in gradient formats 4–15% and 4–20%. Ideal for samples that contain a broad range of protein sizes, the new formats extend Bio-Rad’s line of mini- and midi-sized stain-free precast gels, providing researchers with the fastest separation and in-gel protein visualization capabilities available for Laemmli-based PAGE.

Typical polyacrylamide gel staining methods that follow Coomassie protocols can take four or more hours to complete. The Mini-PROTEAN TGX Stain-Free precast gels include a unique trihalo compound that allows rapid fluorescent detection of proteins in gels or on membranes without the need for staining. When researchers use the Mini-PROTEAN TGX Stain-Free precast gels as part of Bio-Rad’s V3 Western Workflow™, they can achieve complete protein separation, gel imaging, and data analysis in 20 minutes.

Other key benefits of Mini-PROTEAN TGX Stain-Free precast gels in gradient format include:

Greater transfer efficiency — proteins can be transferred onto PVDF or nitrocellulose membranes in as little as 3 minutes with the Trans-Blot® Turbo™ system

Versatility — the same gel can be used for western blotting, standard staining methods, and mass spectrometry analysis of proteins

Cost-effectiveness — unlike other precast gels that require specialized buffer systems and stains, the Mini-PROTEAN TGX Stain-Free precast gels use inexpensive standard Laemmli buffer and do not require staining and destaining, keeping running costs low

The Mini-PROTEAN TGX Stain-Free precast gels are available in a wide range of percentages: 7.5%, 10%, 12%, 4–15%, 4–20%, and Any kD™ (offers optimal resolution in the 10–100 kD range). Gels can be stored for up to 12 months without loss of performance.

For product details or to order Mini-PROTEAN TGX Stain-Free gels, visit http://bit.ly/MP_SFTGX.

A Revolutionary Tool for 5 Minute Protein Visualization and Analysis

 :: Posted by American Biotechnologist on 11-11-2010

A short while ago, Bio-Rad Laboratories (who we all know as experts in electrophoresis and gel documentation) came out with a revolutionary new imaging system that allows for visualization of RNA, DNA and proteins and provides publication-quality images and analysis in seconds — with just the push of a button. (For more reading on the Gel Doc™ EZ system see our previous post on the subject.) Since it’s launch, the Gel Doc™ EZ System has been well received by the research community and is proving to be a darling tool of molecular biologists everywhere.

In the attached technical bulletin, you will learn more about a new technology that dramatically reduces the time required to detect and quantify proteins in a gel, and improves the user’s ability to reproducibly validate an affinity purification procedure.

The paper describes how the Gel Doc EZ Imaging system can be used in combination with stain-free gels to reduce background noise and give superior results for non-quantitative and quantitative gel analysis. This means that bands that could not have otherwise been seen using a standard Coomassie stain can now be visualized with the Criterion Stain Free system.

If you’re interested in protein visualization in polyacrylamide gels within 5 minutes of completing electrophoreic sample separation (no staining, destaining or fiddling with imaging system settings necessary), you should definitely read this bulletin.

Rapid Validation of Purified Proteins using Criterion Stain Free Gels.