SDS-PAGE is a widely used tool for analyzing protein mixtures by electrophoresis. The Laemmli system is regarded as the gold standard for SDS-PAGE techniques due to its ability to resolve complex samples from a wide variety of sources with different buffer backgrounds.
Bio-Rad Laboratories new Mini-PROTEAN TGX (Tris-Glycine eXtended) precast gels are based on a novel modification of the Laemmli system, which significantly increases the gel matrix stability.
The Mini-PROTEAN TGX gels are currently available in a variety of concentrations and exhibits similar migration patterns to the gels you are currently using as indicated in the picture below.
One of the most unique gels in the Mini-PROTEAN TGX series is the Any kD gel which offers optimal resolution of proteins in the 20-100kD molecular weigh range and is ideal for 2-D PAGE.
For more details on the Mini-PROTEAN TGX system including details on:
- easy-to-use cassette design
- performance specs
- sample compatibility
- sample loading tolerance (great lane and band symmetry even when overloaded)
- molecular weight estimation accuracy
- compatibility with downstream applications
- transfer efficiency