Bio-Rad Laboratories recently announced the launch of its Precision Blue real-time PCR dye, making it easier for researchers to set up real-time PCR (qPCR) experiments.
The blue dye improves the visibility of qPCR reaction mixtures, enabling loading of small volume reactions with greater speed, accuracy, and precision. Applications such as single nucleotide polymorphism (SNP) genotyping and high-throughput gene expression analysis using 384-well plates will particularly benefit from use of this dye.
Precision Blue dye is compatible with Bio-Rad’s universal qPCR supermixes (iTaq™ universal and SsoAdvanced™ universal supermixes) and does not interfere with SYBR® Green- or probe-based detection. The dye can be used with all Bio-Rad CFX real-time PCR systems and all other vendors’ real-time PCR systems.
For convenience, Precision Blue can be added directly to the supermix or used as a component of a reaction master mix. The dye is concentrated 200x, and delivers 500 x 20 µl reactions.
For more information about Precision Blue dye, please visit www.bio-rad.com/PrecisionBlue.