:: Posted by American Biotechnologist on 07-15-2014
Bio-Rad Laboratories announced the launch of the SsoAdvanced™ Universal Inhibitor-Tolerant SYBR® Green Supermix, a proven solution for obtaining reproducible, optimal-quality qPCR data from the most challenging samples.
Generating quality qPCR data from difficult-to-amplify samples such as crude lysates from plants, tissues, formalin-fixed paraffin-embedded samples, and other less-than-ideal sources that contain PCR inhibitors can be challenging and frustrating. In these situations, PCR may be greatly inhibited, making it nearly impossible for researchers to obtain reliable data, wasting time and money.
The formulation of the SsoAdvanced™ Universal Inhibitor-Tolerant SYBR® Green Supermix has been optimized and validated to tolerate a wider spectrum of PCR inhibitors than competing SYBR® Green reagents. These inhibitors include those found in crude lysates, polysaccharides and polyphenols, and various reagents left over from sample prep such as ethanol, isopropanol, EDTA, and sodium chloride see performance data for all inhibitors.
As with all SsoAdvanced Supermixes, the inhibitor-tolerant version includes Bio-Rad’s Sso7d-fusion polymerase, which is engineered for enhanced qPCR performance. The supermix is compatible with all real-time PCR instruments and functions under any reaction condition.
Visit www.bio-rad.com/SUIT1 to learn more about SsoAdvanced™ Universal Inhibitor-Tolerant SYBR® Green Supermix.
:: Posted by American Biotechnologist on 07-07-2014
Bio-Rad Laboratories, Inc. announced the launch of its S3™ Biosafety System Class I, an intelligent aerosol containment hood designed for Bio-Rad’s S3™ Cell Sorter. The system adheres to the biosafety requirements of the International Society for Advancement of Cytometry (ISAC) and protects users and the environment from aerosols created during the cell sorting process.
The S3 Biosafety System Class I encloses the S3 Cell Sorter on the benchtop, providing an alternative to the large and expensive biosafety hoods traditionally used with cell sorters.
Additional benefits of the S3 Biosafety System Class I include:
- Software control — integrates with Bio-Rad’s S3™ ProSort™ Software, which provides real-time information about biosafety system status, system temperature, and HEPA filter life
- Fully accessible — offers easy access to the S3 Cell Sorter for cleaning and maintenance via vinyl walls that are magnetically attached to the aluminum biosafety system frame
- Quiet and energy efficient — generates low vibration and sound levels while specialized fans and low backpressure from the HEPA filter reduce energy usage
For more information about Bio-Rad’s S3 Cell Sorter, please visit www.bio-rad.com/cellsorter.
:: Posted by American Biotechnologist on 06-09-2014
DNA methylation is one of the most studied epigenetic modifications. DNA methylation plays an important role in a number of physiological processes as well as common diseases such as cancer and neurodegenerative disorders. Recent data have shown a correlation of DNA methylation and disease status. However, there is an unmet need for a sensitive yet robust method to analyze DNA methylation in clinical samples.
In the bulletin “Droplet Digital PCR: Detection of DNA Methylation”, Bio-Rad Laboratories demonstrates teh unprecedented sensitivity, linearity and robustness of DNA methylation detection by ddPCR in a model system for an imprinted gene involved in neurological disorders.
Read “Droplet Digital PCR: Detection of DNA Methylation” for more information.
:: Posted by American Biotechnologist on 05-29-2014
Publications have become very strict when it comes to publishing research that includes images of blots, often requiring images of the full blot, uncut. To keep the membranes uncut, researchers often reprobe which can cause the marker to fade.
Bio-Rad has launched the brightest protein marker available to researchers which addresses such challenges. The Precision Plus Dual Protein Standard is not only brighter, but also stays on the membrane longer. This allows researchers to identify proteins more effectively and have greater confidence in their western blots.
The strong marker persistence of the Precision Plus Protein™ Dual Color Standards throughout electrophoresis and western blotting, even during rigorous stripping and reprobing protocols, makes the product a more effective tool for monitoring separation and estimating protein molecular weights.
“Providing the images of the entire blot is good practice for science and publications,” said Dr. Federica del Monte, assistant professor of medicine at Harvard Medical School. “In order to reprobe for the loading control and keep the membranes uncut, we sometimes need to strip them. This often results in fading of the markers. The ability to publish clear blots with the marker still on is very important.”
Key benefits of the new Precision Plus Protein Dual Color Standards include:
- Increased brightness — better identification of target proteins on gels and blots as well as easier monitoring of gel electrophoresis and confirmation of transfer quality
- Stronger marker persistence — marker remains on the blot even under the most rigorous conditions, boosting confidence in your western blots; stronger marker persistence also facilitates accurate molecular weight estimation
Bands in the Precision Plus Protein Dual Color Standards range from 10 to 250 kD with sharp pink reference bands at 25 and 75 kD for simple visualization. The new Dual Color Standards share the same migration pattern as their previous iteration.
:: Posted by American Biotechnologist on 05-14-2014
Bio-Rad Laboratories recently announced the launch of its Precision Blue real-time PCR dye, making it easier for researchers to set up real-time PCR (qPCR) experiments.
The blue dye improves the visibility of qPCR reaction mixtures, enabling loading of small volume reactions with greater speed, accuracy, and precision. Applications such as single nucleotide polymorphism (SNP) genotyping and high-throughput gene expression analysis using 384-well plates will particularly benefit from use of this dye.
Precision Blue dye is compatible with Bio-Rad’s universal qPCR supermixes (iTaq™ universal and SsoAdvanced™ universal supermixes) and does not interfere with SYBR® Green- or probe-based detection. The dye can be used with all Bio-Rad CFX real-time PCR systems and all other vendors’ real-time PCR systems.
For convenience, Precision Blue can be added directly to the supermix or used as a component of a reaction master mix. The dye is concentrated 200x, and delivers 500 x 20 µl reactions.
For more information about Precision Blue dye, please visit www.bio-rad.com/PrecisionBlue.