Posts Tagged ‘protein analysis’

From proteins to biosystems

 :: Posted by American Biotechnologist on 03-08-2011

There have been so many incredible tools added to NCBI over the last several years (Reactome, BioSystems, Pathway interaction database) that it can be overwhelming to try and keep up with the latest and greatest NCBI technology.

Less than a year ago, NCBI released FLink (frequency weighted links) which is a tool that enables users to traverse from a group of records in a source database to a ranked list of associated records in a destination database. I found FLink useful for cross-referencing my favorite gene (ETAR) against the BioSystems in which it appears. FLink’s is extremely easy to navigate and in combination with NCBI BioSystems helps to simplify complex processes.

Although FLink is pretty easy to use and self-explanatory, the OpenHelix blog has posted a video tutorial of FLink on SciVee which is a good place to start for anybody wishing to learn more about the tool before actually diving in.

How often have you used FLink? What is your favorite NCBI tool and how are you using it in your research?

A Revolutionary Tool for 5 Minute Protein Visualization and Analysis

 :: Posted by American Biotechnologist on 11-11-2010

A short while ago, Bio-Rad Laboratories (who we all know as experts in electrophoresis and gel documentation) came out with a revolutionary new imaging system that allows for visualization of RNA, DNA and proteins and provides publication-quality images and analysis in seconds — with just the push of a button. (For more reading on the Gel Doc™ EZ system see our previous post on the subject.) Since it’s launch, the Gel Doc™ EZ System has been well received by the research community and is proving to be a darling tool of molecular biologists everywhere.

In the attached technical bulletin, you will learn more about a new technology that dramatically reduces the time required to detect and quantify proteins in a gel, and improves the user’s ability to reproducibly validate an affinity purification procedure.

The paper describes how the Gel Doc EZ Imaging system can be used in combination with stain-free gels to reduce background noise and give superior results for non-quantitative and quantitative gel analysis. This means that bands that could not have otherwise been seen using a standard Coomassie stain can now be visualized with the Criterion Stain Free system.

If you’re interested in protein visualization in polyacrylamide gels within 5 minutes of completing electrophoreic sample separation (no staining, destaining or fiddling with imaging system settings necessary), you should definitely read this bulletin.

Rapid Validation of Purified Proteins using Criterion Stain Free Gels.