A short while ago, Bio-Rad Laboratories (who we all know as experts in electrophoresis and gel documentation) came out with a revolutionary new imaging system that allows for visualization of RNA, DNA and proteins and provides publication-quality images and analysis in seconds — with just the push of a button. (For more reading on the Gel Doc™ EZ system see our previous post on the subject.) Since it’s launch, the Gel Doc™ EZ System has been well received by the research community and is proving to be a darling tool of molecular biologists everywhere.
In the attached technical bulletin, you will learn more about a new technology that dramatically reduces the time required to detect and quantify proteins in a gel, and improves the user’s ability to reproducibly validate an affinity purification procedure.
The paper describes how the Gel Doc EZ Imaging system can be used in combination with stain-free gels to reduce background noise and give superior results for non-quantitative and quantitative gel analysis. This means that bands that could not have otherwise been seen using a standard Coomassie stain can now be visualized with the Criterion Stain Free system.
If you’re interested in protein visualization in polyacrylamide gels within 5 minutes of completing electrophoreic sample separation (no staining, destaining or fiddling with imaging system settings necessary), you should definitely read this bulletin.