Posts Tagged ‘Bio-Plex’

Superior Profiling of Cancer, Cardiovascular, and Other Disease Biomarkers

 :: Posted by American Biotechnologist on 12-15-2014

Bio-Plex Pro Human Inflammation Panels allow simultaneous quantification of 37 key biomarkers of inflammation.

Bio-Plex Pro Human Inflammation Panels allow simultaneous quantification of 37 key biomarkers of inflammation.

Bio-Rad Laboratories announced the launch of its new Bio-Plex Pro™ Human Inflammation Panels, multiplex immunoassays that enable detection and quantification of the most comprehensive set of key inflammation biomarkers in a single assay.

Inflammation contributes to seven of the top ten causes of mortality, including heart disease, cancer, and diabetes. This underscores the critical need to identify and monitor underlying inflammation biomarkers — biochemical signs of inflammation — to aid in disease research and drug discovery and development. Other companies have developed products to quantify inflammation biomarkers, but have thus far not been able to deliver a multiplex solution that allows analysis of key inflammation biomarkers in a single assay.

The Bio-Plex Pro Human Inflammation Panel is the first multiplex immunoassay to enable detection of key inflammation biomarkers from the tumor necrosis factor (TNF), human interferon (IFN), matrix metalloproteinases (MMP), and IL-10/regulatory T cell (Treg) cytokine families in one assay. Using this panel, researchers can now study 37 key biomarkers of inflammation simultaneously in a single assay, allowing them to save precious time, money, and sample.

Benefits of Bio-Plex Pro Human Inflammation Panels include:

  • Flexible ordering options — the panels are available as premixed and ready-to-use large screening kits (37-plex or 24-plex), a pathway-focused Treg 12-plex kit, singleplex assays, or any custom kits
  • Rapid results — multiplex data are generated in 3–4 hours through the use of magnetic beads and protocols that deliver rapid turnaround time
  • Easy preparation — Bio-Plex Pro Kits require only a single sample dilution factor and single-level control, simplifying setup

Bio-Plex Pro Human Inflammation Panels are compatible with the Bio-Plex® 100/200,
Bio-Plex® 3D, and Bio-Plex® MAGPIX™ Platforms, as well as all other Luminex-based xMAP Instruments and Software.

For more information about Bio-Rad’s Bio-Plex product portfolio, please visit www.bio‑rad.com/bioplexpr.

Bio-Rad Introduces Bio-Plex Manager™ MP Software Upgrade for All MAGPIX Multiplex Readers

 :: Posted by American Biotechnologist on 02-20-2014

Bio-Rad Laboratories, Inc. announced the release of its Bio-Plex Manager MP Software Upgrade, which runs on all Luminex-based MAGPIX readers. Users of Bio-Rad’s Bio-Plex® MAGPIX™ instruments have already benefited from the ease of use and improved instrument performance that Bio-Plex Manager MP software brings to multiplex experiments, and now, with Bio-Plex Manager MP Software Upgrade, these benefits are available on all Luminex-based MAGPIX readers.

Over the past decade and a half, life science researchers have relied on Luminex bead-based immunoassays to gain more information from less sample in less time. Bio-Rad’s years of experience with xMAP technology have provided the company with in-depth knowledge and an understanding of the needs of multiplex users, including their desire for automation and a simplified user experience.

The Bio-Plex Manager MP Software Upgrade brings the benefits of Bio-Plex Manager MP software, including automated instrument management, to all Luminex-based MAGPIX readers.


The Bio-Plex Manager MP Software Upgrade brings the benefits of Bio-Plex Manager MP software, including automated instrument management, to all Luminex-based MAGPIX readers.

Bio-Plex Manager MP software offers an automated instrument management function, a unique feature that guides routine maintenance of the instrument based on its current status and performance. This automated feature ensures high quality data and optimal performance by:

  • Automatically loading start-of-day maintenance routines based on instrument status
  • Monitoring performance during data acquisition, alerting the user of performance issues, and resolving those issues
  • Recommending simple stepwise troubleshooting

All of these benefits are now available for all Luminex-based MAGPIX readers through the Bio-Plex Manager MP Software Upgrade.

For more information on Bio-Rad’s Bio-Plex product portfolio, please visit www.bio-rad.com/bioplex.

Quantifying a panel of angiogenic targets in microliters of sample

 :: Posted by American Biotechnologist on 11-10-2011

Angiogenesis is a fundamental process required for multiple physiological and pathological events. It is also a hallmark of over 50 different disease states, including cancer, rheumatoid arthritis, cardiovascular diseases, diabetes and psoriasis. Methods developed to study these diseases are important tools for testing potential therapeutics. These methods include both in vivo and in vitro assays. In vivo assays are considered the most informative because of the complex nature of vascular responses to test reagents. However these assays are often costly and laborious. In contrast, in vitro assays can be carried out expeditiously, are less expensive, and are easier to interpret. Often, these in vitro assays provide maximum benefits when developed as multivariate index assays where the data of multiple assays yield a composite profile of clinically relevant protein biomarkers.

Bio-Rad has developed a multiplex Bio-Plex Pro human cancer biomarker panel that measures angiogenesis biomarker in diverse matrices including serum, plasma, cell culture supernatant and many other sample types. Using this assay, it is possible to quantify multiple angiogenesis targets n a single well of a 96-well microplate in just three hours, using as little as 12.5ul of serum or plasma.

To read more see the attached technical note.

Working Range vs. Dynamic Range in Quantitative Immunoassays

 :: Posted by American Biotechnologist on 06-01-2011

For any quantitative immunoassay, the distinction between the dynamic range of an assay versus working range of an assay is an important consideration. Many believe the working range of an assay and the dynamic range of an assay are one and the same. However, this is not the case. Here we discuss the distinction between these two terms.

For quantitative immunoassays the dynamic range of an assay is described as the lowest to the highest concentration of an analyte that can be reliably detected by the assay. This is sometimes referred to as the lower and upper limits of detection (LLOD and ULOD, respectively). Although signal is detected, the accuracy and precision of this number may vary beyond what is acceptable to report as an accurate measure of the concentration of the target. Although still a useful measure, dynamic range is not as rigorous a measure of the true range of the assay.

For most labs, the working assay range is a more meaningful measure of the upper and lower limits of quantitation (ULOQ/LLOQ) of an assay. The working range of an assay is commonly defined as the range over which analyte concentrations can be quantitated with acceptable precision and reliability. Because this is a stricter measure and requires both sensitivity and accuracy, the working range is typically narrower than the dynamic range. However, it is a more reliable measure of the true range of concentrations that can be accurately quantitated.

As compared to the dynamic range, the values associated with working range of an assay are both precise (defined as how reproducible multiple measurements or calculations are) and accurate (defined as how close a measured or calculated quantity is to its true value).

Bio-Rad Laboratories is a leading provider of instrumention and assays for performing quantitative immunoassays. Bio-Rad’s Bio-Plex instrument is a powerful system for quantitative analysis of up to 100 different proteins, peptides, DNA fragments and RNA fragments from a single drop of sample.

To learn more be sure to view the following Bio-Plex tutorial videos posted right here on the American Biotechnologist.

A powerful system for multiplex analysis

Programming Bio-Plex Manager software

Analyzing Bio-Plex experimental data

Analyzing Bio-Plex experimental data

 :: Posted by American Biotechnologist on 05-05-2011

In this latest video tutorial from Bio-Rad Field Application Specialist Dr. Sean Taylor, you will learn how to analyze your Bio-Plex experimental results using Bio-Plex Manager software.