Posts Tagged ‘Real-Time PCR’

The Next Revolution In PCR

 :: Posted by American Biotechnologist on 03-11-2014

Clearly digital PCR has come a long way in recent years, thanks in large measure to the development of commercial systems like the QX200. These technology advances seem to indicate a tipping point where a greater number of researchers will soon have access to the technology, which will spur development of new applications that take advantage of the full capabilities of digital PCR and move scientists towards more robust biomarker studies and even single cell analyses.

Could the next revolution in PCR be digital? In an article appearing in Biotechniques, Nathan Blow takes a look at the history of digital PCR and why the methodology might have finally reached a tipping point in development.

Read the article to find out:

  • What makes digital PCR different from traditional PCR
  • A short history of digital PCR
  • Why did it take so long for the technology to catch on with developers and researchers?
  • How does digital PCR compare to real-time-quantitative PCR?

To learn more read Digital PCR: Separating from the Pack.

The State of RT-qPCR: Firsthand Observations of MIQE Implementation

 :: Posted by American Biotechnologist on 12-12-2013

Introduction to High Resolution Melt Analysis

 :: Posted by American Biotechnologist on 03-05-2012

In our last post we told you about how Bio-Rad Laboratories very own Sean Taylor and Francisco Bizouarn were crowned the kings of MIQE. Today we’d like to bring you another classic from his majesty Frank. In the slideshow below, you will learn the basics of High Resolution Melt Analysis (HRM), applications, important considerations, assay design and optimization and analysis software. Enjoy. And all hail the king!

Making sure you are in control of your gene expression analysis

 :: Posted by American Biotechnologist on 09-27-2011

Learn which factors affect gene expression analysis and how Bio-Rad Laboratories can help.

Increase the amount of data from your RNA samples

 :: Posted by American Biotechnologist on 08-24-2011

Some of the technical challenges in qPCR research can be traced back to the reverse transcription step and the synthesis of cDNA for qPCR analysis.

Limitations in the maximum amount of template RNA that can be added to an RT reaction make it tougher to detect low-abundant target genes and limits the amount of data that can be obtained from a typical 20ul RT reaction.

Bio-Rad Laboratories has recently launched the iScript advanced cDNA sythesis kit for RT-qPCR as an answer to some of these challenges.

iScript advanced is a 2-tube format cDNA synthesis kit with a short protocol (35 minutes) and an allowed RNA input amount of up to 7.5ug.
The kit will:

  • increase the amount of data that can be obtained from an RT reaction (great for high-throughput labs)
  • enable you to analyze a larger number of target genes from a single sample
  • increase the sensitivity for certain target genes due to higher RNA input capacity
  • cover a broad dyamic range and sensitivity

For more information on iScript Advanced including experimental data download the iScript Advanced product bulletin.

To read more about Bio-Rad’s qPCR solutions visit