Posts Tagged ‘gene expression studies’

Simplifying Gene Knockdown Experiments

 :: Posted by American Biotechnologist on 05-17-2010

If you’ve ever done gene knockdown experiments, you know that the process can be complicated and involve many steps. siRNA design, cell culture, perfect cell confluency, transfection or electroporation, downstream analysis etc. Bio-Rad now has a plethora of instruments and reagents to help you simplify the process. In a recently published article in the Journal of Visualized Experiments, scientists demonstrated a pathway study that makes use of Bio-Rad’s many strengths in the area of cell culture and gene silencing including streamlining cell counting with the TC10 automated cell counter, electroporating multiple samples simultaneously using the MXCell electroporation system and simultaneously assessing RNA quality and quantity with the Experion automated electrophoresis system.

If you want to learn about more ways to simplify your siRNA experiments checkout:

McCoy, A., Litterst, C., Collins, M., & Ugozzoli, L. (2010). Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells Journal of Visualized Experiments (38) DOI: 10.3791/1904

The Wonders of Automation

 :: Posted by American Biotechnologist on 05-12-2010

Bio-Rad’s latest gadget is the TC10 automated cell counter, which accurately and reproducibly provides total mammalian cell counts in 30 seconds or less—eliminating the need for manually counting cells under the microscope using a hemocytometer.

The TC10 cell counter completes the counting of mammalian cells—suspended or re-suspended adherent—in one simple step, initiating a count immediately upon slide insertion. Unlike other cell counting devices, which may draw inaccurate conclusions from using a single plane of focus to assess cell viability, the TC10 cell counter scores each sample across multiple planes. The unique auto-focus technology and image analysis algorithm provide accurate and reproducible cell counts in less than 30 seconds for cell concentrations between 5 x 104 and 1 x 107 cells/ml and cells within a 6–50 µm diameter range. Since only 10 µl of suspended cells is required for counting, precious sample is conserved.

Cell viability can also be assessed with the TC10 cell counter with the use of trypan blue, a dye which is excluded from live cells and incorporated into dead cells. The system recognizes the presence of trypan blue without user intervention and provides both cell count and cell viability, in less than 30 seconds.

The TC10 cell counter saves time with its on-board dilution calculator which can determine the volume adjustments needed to achieve the cell concentration for the next passage or experiment. In experiments using trypan blue, the dilution calculation is based on the number of live cells.

Using the USB port, researchers can export annotated JPEG image files of their samples for further analysis or download counting data in a spreadsheet compatible format. Results can also be printed directly onto labels using the separate TC10 printer.

For an online demo of how to use the TC10 and other video tutorials including the TC10′s use in gene expression studies, a cell biology primer and a basic cell culture introductory document visit the TC10 website.