How to Avoid Unstable Results When Performing High-Throughput qPCR

If your biotechnology research requires you to be engaged in high-throughput Quantitative PCR analysis than you’ve probably made good use out of automated systems such as liquid handling robots and plate stackers. And what a relief they are. Imagine what your poor thumb would feel like if you had to pipette out tens of thousands of reactions. Or think about how much time you would have wasted standing around waiting for a run to end in order to load the next plate into the QPCR machine.

While automated handing systems are fantastic, they also bring with them the complicated question of sample stability. When samples are loaded into the automated system, they may be stored there at room temperature for hours. As such, it is important for the reaction mix to be stable at room temperature for the duration of the waiting period.

In this Bio-Rad Technical note, you will learn how Bio-Rad’s SsoFast EvaGreen supermix meets the challenge of providing reaction mix stability and can be used to acheive consistent results over a large dynamic range of templates with automated runs lastng up to 48 hours.

Stability for Automated qPCR

Tags: , ,

Leave a Reply