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Download the Protein Blotting Guide
Download the Stem Cell Guide for Life Science Researchers
:: Posted by American Biotechnologist on 02-27-2013
Needless to say, unbiased reviews of products on corporate websites are often hard to come by. However, if you are in the market for new equipment, Biocompare has a wide selection of unbiased reviews on numerous products that are worth reading before you purchase.
Recently, a scientists at the Cancer Center in the Sanford/Burnham Medical Research Instituted, wrote a review of Bio-Rad Laboratories Trans-blot Turbo System. In his 7 point review, Dr. Dayong Zai provided some useful tips such as the importance of getting rid of all the bubbles prior to transfer and cleaning the system thoroughly once the transfer has finished. The review gave teh Trans-blot Turbo thumbs up for its ease of use, fast transfer times and reproducible results and a thumbs down for the bad smell produced once the transfer has completed.
:: Posted by American Biotechnologist on 05-22-2012
Power supplies that are used for electrophoresis hold one parameter constant (either voltage, current, or power). The PowerPac™ HC and PowerPac Universal power supplies also have an automatic crossover capability that allows the power supply to switch over to a variable parameter if a set output limit is reached. This helps prevent damage to the transfer cell.
During transfer, if the resistance in the system decreases as a result of Joule heating, the consequences are different and depend on which parameter is held constant.
Transfers Under Constant Voltage
If the voltage is held constant throughout a transfer, the current in most transfer systems increases as the resistance drops due to heating (the exception is most semi-dry systems, where current actually drops as a result of buffer depletion). Therefore, the overall power increases during transfer, and more heating occurs. Despite the increased risk of heating, a constant voltage ensures that field strength remains constant, providing the most efficient transfer possible for tank blotting methods. Use of the cooling elements available with the various tank blotting systems should prevent problems with heating.
Transfers Under Constant Current
If the current is held constant during a run, a decrease in resistance results in a decrease in voltage and power over time. Though heating is minimized, proteins are transferred more slowly due to decreased field strength.
Transfers Under Constant Power
If the power is held constant during a transfer, changes in resistance result in increases in current, but to a lesser degree than when voltage is held constant. Constant power is an alternative to constant current for regulating heat production during transfer.
:: Posted by American Biotechnologist on 05-15-2012
The results are in from SelectScience’s Scientists’ Choice Award and Bio-Rad Laboratories‘ Trans-Blot® Turbo™ has taken the prize for Best Life Science Product of 2011. Over 30,000 SelectScience members were invited to vote for their favorite product of 2011 and the Trans-Blot Turbo was given the recognition that it deserves.
Click on the picture below to watch the award ceremony.
:: Posted by American Biotechnologist on 10-17-2011
You could win the Grand Prize! Simply complete the online form and you will be automatically entered into a prize draw to win a package of electrophoresis equipment and reagents for a complete year’s electrophoresis!
What Do I Win?
Imagine winning the whole suite of products needed for electrophoresis?
Grand Prize: ChemiDoc™ MP imaging system, 4 Immunstar™ Western C™ Chemiluminescent kits, Trans-Blot Turbo® system, 300 transfer packs, and 2 packages of the 5-pack Precision Plus Protein™ Dual Color Standards, Mini-PROTEAN® Tetra Cell and 300 Mini-PROTEAN TGX™ Stain-Free gels, Laemmli Sample Buffer and Running Buffer, PowerPac Basic™ Power Supply (100–120/220–240 V)
Second Place Prize: (3 sets) 1 Trans-Blot Turbo system, 300 Trans-Blot Turbo transfer packs and protein standards; 1 Mini-PROTEAN Tetra Cell and 300 Mini-PROTEAN TGX gels, Laemmli sample buffer, 2 packages of 5-Pack Precision Plus Protein Dual Color Standards, and 4 Immun-Star WesternC Chemiluminescent Kits
Third Place Prize: 50 Mini-PROTEAN TGX shirts
Prizes announced on February 1, 2012. Click here for details.