Using Bio-Rad’s TC10 automated cell counter is a breeze! Watch this video and learn how to use the TC10 counter to count cells in less than 30 seconds before splitting them or running an experiment. He discusses the innovative auto-focus technology and how it helps to increase accuracy and reproducibility.
Posts Tagged ‘TC10 Cell Counter’
For over 100 years the hemocytometer has been used by cell biologists to quantitate cells. It was first developed for the quantitation of blood cells but became a popular and effective tool for counting a variety of cell types, particles and even small organisms. Currently, hemocytometers, armed with improved Neubauer grids, are a mainstay of cell biology labs.
Despite its longevity and versatility, hemocytometer counting suffers from a variety of shortcomings. These shortcomings include, but are not limited to, a lack of statistical robustness at low sample concentration, poor counts due to device misuse, and subjectivity of counts among users, in addition to a time-consuming and tedious operation. In recent years automated cell counting has become an attractive alternative to manual hemocytometer-based cell counting, offering more reliable results in a fraction of the time needed for manual counting.
The attached report compares the precision of cell counts obtained with a hemocytometer to hose obtained by automated cell counting using the Bio-Rad TC10 automated cell counter. Sources of error that are inherent to the device, and those introduced by the operator are investigated. We demonstrate that automated cell counting can significantly reduce user and concentration-dependent count variance, while greatly reducing the time needed to perform counts.
Bio-Rad’s latest gadget is the TC10 automated cell counter, which accurately and reproducibly provides total mammalian cell counts in 30 seconds or less—eliminating the need for manually counting cells under the microscope using a hemocytometer.
The TC10 cell counter completes the counting of mammalian cells—suspended or re-suspended adherent—in one simple step, initiating a count immediately upon slide insertion. Unlike other cell counting devices, which may draw inaccurate conclusions from using a single plane of focus to assess cell viability, the TC10 cell counter scores each sample across multiple planes. The unique auto-focus technology and image analysis algorithm provide accurate and reproducible cell counts in less than 30 seconds for cell concentrations between 5 x 104 and 1 x 107 cells/ml and cells within a 6–50 µm diameter range. Since only 10 µl of suspended cells is required for counting, precious sample is conserved.
Cell viability can also be assessed with the TC10 cell counter with the use of trypan blue, a dye which is excluded from live cells and incorporated into dead cells. The system recognizes the presence of trypan blue without user intervention and provides both cell count and cell viability, in less than 30 seconds.
The TC10 cell counter saves time with its on-board dilution calculator which can determine the volume adjustments needed to achieve the cell concentration for the next passage or experiment. In experiments using trypan blue, the dilution calculation is based on the number of live cells.
Using the USB port, researchers can export annotated JPEG image files of their samples for further analysis or download counting data in a spreadsheet compatible format. Results can also be printed directly onto labels using the separate TC10 printer.
For an online demo of how to use the TC10 and other video tutorials including the TC10’s use in gene expression studies, a cell biology primer and a basic cell culture introductory document visit the TC10 website.