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Download the Protein Blotting Guide
Download the Stem Cell Guide for Life Science Researchers
:: Posted by American Biotechnologist on 11-12-2009
Bio-Rad Laboratories has put together a great tutorial on QPCR analysis including a thorough review of what the graphs mean and an overview of Absolute and Relative methods of quantification. The tutorial also includes a great explanation of the differences between Livak, delta CT and the Pfaffl methods of analyzing your results.
Watch part II: normalization against a reference gene
:: Posted by American Biotechnologist on 10-27-2009
There has been a lot of buzz lately on the newly published guidelines detailing the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines published by Bustin et al. in Clinical Chemistry 55:4, 2009. The paper was coauthored by several important PCR authorities including Jo Vandesompele and is therefore sure to become an important standard for real-time PCR publications. Dr. Sean Taylor of Bio-Rad has put together several short youtube videos explaining the importance of MIQE and how it can be applied to the bench.
:: Posted by American Biotechnologist on 10-16-2009
Nucleic acid amplification and detection are among the most valuable techniques used in biological research today. Scientists in all areas of research — basic science, biotechnology, medicine, forensic science, diagnostics, and more — rely on these methods for a wide range of applications. For some applications,
qualitative nucleic acid detection is sufficient. Other applications, however, demand a quantitative analysis. Choosing the best method for your application requires a broad knowledge of available technology.
This guide from Bio-Rad provides an introduction to many of the technical aspects of real-time PCR. It includes guidelines for designing the best real-time PCR assay for your experiments and explains how real-time PCR data are used in various applications.