:: Posted by American Biotechnologist on 03-23-2010
The tech note that was written last year entitled: “A practical approach to RT-qPCR- Publishing data that conform to the MIQE guidelines” by Sean Taylor, Michael Wakem, Greg Dijkman, Marwan Alsarraj and Marie Nguyen has been published in Methods (2010 Apr;50(4):S1-5.).
Taylor S, Wakem M, Dijkman G, Alsarraj M, & Nguyen M (2010). A practical approach to RT-qPCR-Publishing data that conform to the MIQE guidelines. Methods (San Diego, Calif.), 50 (4) PMID: 20215014
To support the MIQE Guidelines and best practices for qPCR experiments, Bio-Rad has initiated a half-day qPCR workshop to:
1. Analyze your own RNA samples to assure that they are of appropriate quality and purity for qPCR.
2. Demonstrate the importance of accurate pipetting, good technique and quality reagents with a pipetting competition (prizes will be awarded!).
3. Teach you how to design a solid experimental approach for excellent results that conform to the recently published MIQE guidelines (Bustin et al. 2009; Taylor et al. 2010).
If you are interested in holding a workshop in your area please send an email to firstname.lastname@example.org. Be sure to leave your name, email address, phone number and location and someone will get back to you shortly.
:: Posted by American Biotechnologist on 02-16-2010
If you are currently doing quantitative PCR (qPCR) or plan on beginning qPCR experiments you MUST check out this latest video tutorial from Bio-Rad’s tech support group.
This tutorial describes the characteristics of an optimized SYBR green I quantitative PCR (qPCR) assay. qPCR assays must be optimized to ensure results that are biologically and statistically significant. Topics include a brief review of qPCR chemistry, with an emphasis on SYBR Green I reactions, and definitions of the four main characteristics, or hallmarks of an optimized qPCR assay.
:: Posted by American Biotechnologist on 01-19-2010
High-Resolution Melting Analysis (HRMA) is rapidly becoming a very attractive tool to not only to screen patients for pathogenic variants but also for presequence screening, single nucleotide polymorphism (SNP) typing, methylation analysis, quantification (copy number variants), an alternative to gel-electrophoresis and clone characterization. The ease of use, simplicity, flexibility, low cost, nondestructive nature, superb sensitivity, and specificity of HRMA has brought the technique to the forefront of life science research.
I did some literature searching and have attached a recent review that covers the topic very well. The article was written prior to Bio-Rad’s launch of our own HRMA software module integrated into the CFX96 and CFX384 instruments so I have also attached a flyer on the topic which will show you the accessibility to HRMA through these instruments, associated software and SsoFast EvaGreen Supermix.
High Resolution Melt Analysis – Review Article 2009
Precision Melt Analysis Software Bulletin_5798A
Sean Taylor, pHD
Field Applications Specialist
:: Posted by American Biotechnologist on 01-07-2010
As seen in Genomeweb this week, Bio-Rad Laboratories launched an automated front end for its CFX96 and CFX384 real-time PCR detection systems, marking the company’s first automated real-time PCR system. The new technology, called the CFX automation system, includes a bench-top plate handler that can load up to 20 384-well plates, or 7,680 samples, at one time on the CFX384. It also includes control software that manages the configuration and operation of the CFX automation system so that researchers can assign either the same or manifold PCR protocols across each plate. The automated system is targeted at anyone who wants to increase their throughput and run their system in an automated environment.
Essentially, we are talking about having a set of machines that will pipette samples and mastermix into the PCR plate, load the plate into the PCR machine, run the protocol and EMAIL THE DATA to you once the run has completed.
Remember the days of pipetting into centrifuge tubes, setting up 3 waterbaths (one for denaturing, another for annealing and another for extending) and then manually incubating the tubes in each bath for 1 minute and cycling that 30-40 times? (OK…so I’m not that old to remember that either but my master’s degree supervisor kept talking about how he did that when he was in the lab). In any event, doing research used to mean that we’d have to be in the lab setting up our experiments and waiting for our results. How cool is it that I can now set up my reagents, primers probes and samples, walk away, go home and do my analysis from home once the data file is sent to me? This is definitely the wave of the future. Couple this with a laboratory management program such as bioKM from Biodata that could organize your data within the context of your experiment and all you’d be missing is the automatic “peer-reviewed” paper writing machine.
Very cool. What will they think of next!
:: Posted by American Biotechnologist on 12-29-2009
Genomics is rapidly expanding beyond merely identifying the genetic makeup of organisms to encompass studies of how variations in genotype impact physiological functions. See the article below which was published in the October 15th edition of Genetic Engineering and Biotechnology news to learn more about high-res met analysis as an alternative to probe-based genotyping.
High-Resolution Melt Analysis Applications