Posts Tagged ‘qPCR’

More Quantitative Than qPCR

 :: Posted by American Biotechnologist on 05-09-2012

The introduction of real time PCR (qPCR) revolutionized the world of molecular biology and provided scientists with a tool for obtaining truly quantitative nucleic acid data. While qPCR remains one of the most robust tools available on the bench, its resolution power is limited to changes in DNA concentration that are higher than 50%. This limitation is mainly due to the compounding of errors derived from each step in the quantification process. That’s where Droplet Digital PCR enters the picture.

Droplet Digital PCR alleviates the compounding error effect by partitioning samples into 20,000 droplets such that each copy of DNA is encapsulated in its own bubble. Subsequent amplification reactions are then carried out individually which leads to reduce background noise and therefore a more sensitive measurement of low concentrations of nucleic acid that may not have been detectable using qPCR.

Bio-Rad Laboratories‘ Frank Bizouarn recently published an outstanding article in Genetic Engineering and Biotechnology News detailing the benefits of ddPCR including a real life example. The article covers how ddPCR works and its use in gene-expression analysis, copy number variation determination and rare event detection.

Check out Digital PCR: Improving Nucleic Acid Quantification.

Great video explanation of droplet digital PCR

 :: Posted by American Biotechnologist on 03-21-2012

The QX100 Droplet Digital PCR system from Bio-Rad Laboratories is the third generation of PCR technology. The system provides an absolute measure of target DNA molecules with unrivaled performance in precision, accuracy, and sensitivity for quantitative PCR applications. Watch the video below to learn more about this powerful technology.

Related posts:
Introducing the 3rd Generation of PCR
Video Introduction to Droplet Digital PCR
An Advanced Look at Droplet Digital PCR

Introduction to High Resolution Melt Analysis

 :: Posted by American Biotechnologist on 03-05-2012

In our last post we told you about how Bio-Rad Laboratories very own Sean Taylor and Francisco Bizouarn were crowned the kings of MIQE. Today we’d like to bring you another classic from his majesty Frank. In the slideshow below, you will learn the basics of High Resolution Melt Analysis (HRM), applications, important considerations, assay design and optimization and analysis software. Enjoy. And all hail the king!

More on Bio-Rad’s QX100 Droplet Digital PCR System

 :: Posted by American Biotechnologist on 02-13-2012

The QX100 Droplet Digital PCR system from Bio-Rad Laboratories provides an absolute measure of target DNA molecules with unrivaled precision and accuracy. The QX100 system partitions each sample into 20,000 individual nanoliter-sized droplets. PCR-positive and PCR-negative droplets from every sample are then counted to provide absolute target quantification in digital form. The QX100 system provides a revolutionary approach to target DNA quantification.

The QX100™ ddPCR™ system lets you:

  • Detect rare target sequences with unmatched sensitivity for cancer and viral research
  • Measure small differences in target copy number variations (down to 1.2x differences)
  • Determine absolute gene expression levels without a standard curve or the ΔΔCq method

Click here to download the latest product information, or here to see performance data, copy number variation data, gene expression data and rare mutation detection data.

Related posts:
Introducing the 3rd Generation of PCR
Video Introduction to Droplet Digital PCR
An Advanced Look at Droplet Digital PCR

An Advanced Look at Droplet Digital PCR

 :: Posted by American Biotechnologist on 12-07-2011

As a follow up to our video introduction to droplet digital PCR we are proud to present you with an advanced video presentation on droplet digital PCR technology. Please have a look and let us know what you think!