In our last post we told you about how Bio-Rad Laboratories very own Sean Taylor and Francisco Bizouarn were crowned the kings of MIQE. Today we’d like to bring you another classic from his majesty Frank. In the slideshow below, you will learn the basics of High Resolution Melt Analysis (HRM), applications, important considerations, assay design and optimization and analysis software. Enjoy. And all hail the king!
Posts Tagged ‘qPCR’
The QX100 Droplet Digital PCR system from Bio-Rad Laboratories provides an absolute measure of target DNA molecules with unrivaled precision and accuracy. The QX100 system partitions each sample into 20,000 individual nanoliter-sized droplets. PCR-positive and PCR-negative droplets from every sample are then counted to provide absolute target quantification in digital form. The QX100 system provides a revolutionary approach to target DNA quantification.
The QX100™ ddPCR™ system lets you:
- Detect rare target sequences with unmatched sensitivity for cancer and viral research
- Measure small differences in target copy number variations (down to 1.2x differences)
- Determine absolute gene expression levels without a standard curve or the ΔΔCq method
As a follow up to our video introduction to droplet digital PCR we are proud to present you with an advanced video presentation on droplet digital PCR technology. Please have a look and let us know what you think!
As a follow up to Bio-Rad’s acquisition of QuantaLife, we are proud to present the following educational training video: “An Introduction to Droplet Digital PCR”, given by award winning field application specialist Dr. Sean Taylor.
Be sure to stay tuned for the advanced training video which will be posted in the near future.
I found this nice, down to earth explanation of the delta-delta Ct method for calculating qPCR results (i.e. qPCR results corrected with internal controls).