Posts Tagged ‘PCR’

Meet the T100 thermal cycler from Bio-Rad Laboratories

 :: Posted by American Biotechnologist on 07-19-2011

Intuitive touch screen simplifies PCR

 :: Posted by American Biotechnologist on 07-07-2011

Bio-Rad Laboratories, Inc. introduces the T100 thermal cycler, a compact, reliable, and economical 96-well PCR instrument with an easy-to-use graphical touch screen.

The T100 thermal cycler is ideally suited for small labs or for scientists who prefer a personal instrument on their bench.

Bio-Rad developed the user interface for the T100 thermal cycler so researchers are able to edit, run, and save protocols with ease. The system also provides a way for researchers to stay organized by either storing protocols in personalized folders or exporting them to a USB flash drive.

In addition, researchers can expedite their experiments by using the thermal gradient technology on the T100 thermal cycler to optimize assay conditions. The thermal cycler can test eight annealing temperatures simultaneously to determine the optimal temperature for high reaction specificity and yield.

The T100 thermal cycler provides accurate and uniform heating for sample volumes between 1 and 100 µl, which leads to consistent results. Complete hermetic sealing of the thermoelectric components ensures long-lasting performance.

With the T100 thermal cycler, researchers can:

•Save time programming — An intuitive graphical touch screen makes programming protocols and performing PCR experiments easy.

•Get optimal results faster — Thermal gradient enables reaction optimization in a single run, and with a 4oC/sec maximum ramp rate, runs can be completed quickly.

•Be confident in results — Robust seal protects thermoelectric components from condensation and ensures long-lasting performance.

To learn more about the T100 thermal cycler, visit:

Applications of MIQE to Real Time Quantitative PCR

 :: Posted by American Biotechnologist on 05-24-2011

In this video, Dr. Sean Taylor, Field Applications Specialist, Bio-Rad Laboratories, demonstrates how sample quality and reference gene selection effect data analysis and interpretation in real-time quantitative PCR (qPCR) experiments. The presentation is in accordance with the previously published MIQE guidelines.

For enhanced viewing, click on the full-screen mode button on the bottom right hand corner of the video.

Discovering PCR on the road to Mendocino

 :: Posted by American Biotechnologist on 02-20-2011

What’s the connection between PCR and Mendocino, California? Everyone knows that Kerry Mullis was awarded the Nobel Prize in Chemistry in 1993 for his improvement of PCR, but did you know that his methodology was hatched on the road to his cottage in Mendocino?

Check out the video below to hear the story from Mullis himself.


 :: Posted by American Biotechnologist on 01-27-2011