:: Posted by American Biotechnologist on 02-10-2014
High purity protein is a common requirement for biochemical and structural studies. A common approach is to recombinantly express an affinity-tagged version of the protein of interest. This is, however, not always a viable option. Some proteins are unstable or inactive once tagged or require posttranslational modifications that do not permit recombinant expression. In these cases, researchers often settle for lower purity protein rather than exhaustively explore purification options, since the purification optimization process can be time and labor intensive when no particular column resins or buffer conditions are dictated by an affinity tag.
Bio-Rad Laboratories has recently released a study demonstrating how to purify untagged protein to high homogeneity without undergoing laborious manual troubleshooting steps. Bio-Rad’s ChromLab software can be programmed to execute several runs sequentially thereby automating and accelerating this tedious process.
To learn how to purify untagged protein with ease read Protein Purification Workflow Development Using Bio-Rad’s NGC™ Chromatography System.
:: Posted by American Biotechnologist on 10-07-2013
“It looked like a Medusa,” said Farah Mavandadi. “I was scared of it.”
Mavandadi, a senior product manager at Bio-Rad, was not describing a monster in a nightmare or a creature seen from a diving bell, but rather a research-scale chromatography system. Sprawling over a lab bench, instead of snakes the system had bunches of electronic cables and fluidic tubing that writhed and tangled around its various components.
Despite resembling an unholy mashup between Rube Goldberg and Jason and the Argonauts, this system was highly valued by the researcher who had wrangled it together, lovingly making each complex connection and hand-coding each component into the analytical software. The reason? Flexibility. Despite its messiness, the researcher could rearrange her system to get the most efficient fluidic path, to have right kind of detector in place, or make any other tweak in order to perform the best possible separation. Between having an “easy” instrument and having one she could change as her chromatography needs required, this researcher chose to face “Medusa.”
:: Posted by American Biotechnologist on 04-25-2013
Dr. Christopher Fraser and the members of his lab in the Department of Molecular and Cellular Biology at the University of California, Davis, want to better understand the fundamental cellular process of protein synthesis. The genomic DNA in each cell of an organism encodes the same set of genes, but gene expression is regulated so that only certain proteins are produced at appropriate times in each cell, resulting in diverse cellular functions. The control of the first step of protein synthesis is the regulation of DNA transcription into mRNA. The next step, the translation of mRNA into protein by the ribosome, is also tightly controlled in normal cells, and dysfunctional regulation is responsible for disorders such as autism, Type I diabetes, and cancer.