Posts Tagged ‘mass spectrometry’

An Ideal Fluorescent Stain for Proteomics Research

 :: Posted by American Biotechnologist on 03-16-2010

In order to be useful in proteomics applications, a fluorescent stain must be sensitive enough to visualize protein quantities down to 1 ng or less, exhibit minimal protein-to-protein variability in staining intensity and fully compatible with 2D electrophoresis and mass spectrometric fingerprinting.

In this study, the performance of Bio-Rad’s Oriole gel stain is characterized with respect to its spectral properties, detection sensitivity, protein-to-protein variability and compatibility with mass spectrometry. Furthermore, the study describes how Oriole may be imaged using UV light which is ideal considering that UV-based imaging equipment is relatively simple and inexpensive and tends to be more generally available to laboratory researchers.

Oriole Fluorescent Gel Stain: Characterization with SYPRO Ruby Gel Stain