Posts Tagged ‘Ludesi’

Quality controlling 2D gel data and why you should do it

 :: Posted by American Biotechnologist on 05-31-2010

Be sure to check out the upcoming webinar on Wednesday June 9th from our friends at Ludesi.

Learn how to use the free Gel IQ tool to measure the correctness of your image analysis output.

* Introducing you to Combined Correctness: a generic metric for calculating a quality score
* Using the free software tool “Gel IQ” to calculate a quality score on your 2D gel image analysis data
* How to import your analysis project into Gel IQ
* Applying the correct evaluation settings
* Evaluating spot detection and matching correctness
* Interpreting your Combined Correctness score
* Using Gel IQ to quality control and optimize your 2D gel analysis protocols

Wednesday, June 9, 2010
12 PM EDT / 9 AM PDT (US) / 6 PM CET (Europe)
Click here to register for the Wednesday time slot

Proteomics Refined

 :: Posted by American Biotechnologist on 05-21-2010

As a proteomics fan in general and a 2D Gel Electrophoresis fan specifically, I have been following the Ludesi Blog which I have found to be a resource rich in 2D information including a knowledgebase, webinars and an great tool that pulls together all proteomics related tweets from twitter. For those of you who are not familiar with Ludesi, they are a Swedish company that was founded in 2001 by Ola Forsstrom-Olsson who was listed in MIT Technology Review’s Top 100 list of the World’s Best Technology Innovators Under 35 in 2004.

In addition to having a great blog, Ludesi produces fantastic 2D gel imaging software and provides an analytical service that is fast, user friendly, compatible with all types of stains, has great technical support and a unique pay as you go system. But the best of all is that the experts at Ludesi analyze the gels for you and save you some serious computer time!

To get started:

– Download REDFIN software at www.expressionproteomics.com/redfin and follow the registration
procedure
– Upload 2-D gel images and purchase credits.
– Each gel is analyzed by multiple professionals at Ludesi.
– Data and gels are stored on the redfin site and can be accessed via the secure server from any computer (anywhere) at any time.
– Since Redfin is a Software As A Service (SAAS) it is automatically updated every time you log in (no need to pay for updates
– Best of all…you only PAY FOR WHAT YOU USE!

For a limited time, Bio-Rad has arranged for new customers to receive three free credits when first trying out the service.

What does that mean? Well, to have a gel analyzed using the basic service (appropriate for almost all researchers) you will need 2 credits and to analyze a gel using the Pro service (removes streaks, and artifacts and expands upon quality assurance for spot detection and matching) you will require 10 credits per gel.

To get your free credits, download redfin from www.expressionproteomics.com/redfin, click on the “use value code” button and enter “TRYREDFIN” and away you go!

To read more about Redfin, see the a href=’http://www.americanbiotechnologist.com/blog/wp-content/uploads/2010/05/Redfin-brochure.pdf’>Redfin brochure

For a thorough review on the limits, benefits, and perspectives of gel-based proteomic approaches see the recently published review article in Proteome Science.

Chevalier, F. (2010). Highlights on the capacities of “Gel-based” proteomics Proteome Science, 8 (1) DOI: 10.1186/1477-5956-8-23