Posts Tagged ‘electrophoresis’

Five great tips for proteomic researchers

 :: Posted by American Biotechnologist on 11-03-2011

Here are some great application tips from Bio-Rad Laboratories for those researchers working with proteins:

  • Generally, the best method for keeping a protein in solution is to add any combination of nonionic detergents, zwitterionic detergents, and chaotropic agents to the sample mixture. Also use reducing agents such as DTT and DTE (less than 20 mM) to decrease disulfide bond formation between proteins.

  • When working with membrane or insoluble proteins, increase the amount of SDS in the equilibration and running buffers (up to 0.2%) to allow the proteins to effectively migrate out of the IPG strip.

  • To reduce the amount of SDS in samples generated by preparative SDS-PAGE, substitute the elution buffer with one that does not contain SDS.

  • Nucleic acid contamination is a common cause of horizontal gel streaking. Treat samples with nucleases to remove nucleic acids prior to isoelectric focusing.

  • Never heat samples in urea-containing buffers. The urea rapidly breaks down to carbamic acid and carbamylates the proteins, modifying their charge. Urea breakdown and subsequent protein carbamylation is the cause of charge trains on 2-D gels. A charge train is a series of spots on a 2-D gel that are of different pIs and the same size.

For more great tips visit www.expressionproteomics.com

Enter to Win Electrophoresis for a Year!

 :: Posted by American Biotechnologist on 10-17-2011

You could win the Grand Prize! Simply complete the online form and you will be automatically entered into a prize draw to win a package of electrophoresis equipment and reagents for a complete year’s electrophoresis!

What Do I Win?
Imagine winning the whole suite of products needed for electrophoresis?

  • Grand Prize: ChemiDoc™ MP imaging system, 4 Immunstar™ Western C™ Chemiluminescent kits, Trans-Blot Turbo® system, 300 transfer packs, and 2 packages of the 5-pack Precision Plus Protein™ Dual Color Standards, Mini-PROTEAN® Tetra Cell and 300 Mini-PROTEAN TGX™ Stain-Free gels, Laemmli Sample Buffer and Running Buffer, PowerPac Basic™ Power Supply (100–120/220–240 V)
  • Second Place Prize: (3 sets) 1 Trans-Blot Turbo system, 300 Trans-Blot Turbo transfer packs and protein standards; 1 Mini-PROTEAN Tetra Cell and 300 Mini-PROTEAN TGX gels, Laemmli sample buffer, 2 packages of 5-Pack Precision Plus Protein Dual Color Standards, and 4 Immun-Star WesternC Chemiluminescent Kits
  • Third Place Prize: 50 Mini-PROTEAN TGX shirts

Prizes announced on February 1, 2012. Click here for details.

Improving the statistic significe of your Western Blots

 :: Posted by American Biotechnologist on 09-08-2011

Western Blotting is probably one of the most ubiquitous techniques in the molecular biology lab and relatively easy to perform. Yet many of us have been frustrated with statistically insignificant results or protein bands that appear either too dark or too light to quantitate.

Well, do not despair! There are many things you can do to help improve the quality of your blots and increase your likelihood of obtaining statistically significant results!

In the video below, you will learn about the many factors affecting western blot analysis (such as detection limit and dynamic range limitations of film and overloaded gels) and what can be done to improve your chances of success.

The presentation was given by Bio-Rad Laboratories Field Application Specialist Dr. Sean Taylor as part of an intimate customer training. Some of the references in the presentation may be specific for that particular customer but the general information contained in this presentation is highly valuable to all molecular biology labs.

Still stuck on tank or semi-dry protein blotting?

 :: Posted by American Biotechnologist on 08-17-2011

Learn why the Trans Blot Turbo is faster than tank and semi-dry blotting techniques.

Bio-Rad Video 5/11/11 from Lab Manager Magazine on Vimeo.

A Scientific Legend’s Approach to Solving Problems and Developing Technologies

 :: Posted by American Biotechnologist on 07-26-2011

George Church is one of the founders of the human genome project and continues to play an important role in the personal genome project, stem cell research and biofuel research. In this video, Dr. Sriram Kosuri, a Postdoctoral fellow in George’s lab at Harvard University discusses the Church lab’s approach to solving problems and developing technologies.