It’s no surprise that Droplet Digital PCR (ddPCR) has found its place in the world of cancer research. Bio-Rad’s ddPCR™ technology has a remarkable ability to quantify miniscule amounts of target DNA and RNA, and thus can contribute to early detection of rare tumorigenic mutations against a high background of “normal” DNA as well as to other applications, including identifying cancer subtypes, optimizing drug treatment plans, and studying tumor evolution. At the Fred Hutchinson Cancer Research Center in Seattle, scientists are producing some of the most important advances in prevention, early detection and treatment of cancer and other diseases. They are studying the disease process from every angle seeking to uncover factors that influence a person’s likelihood of getting cancer. Understanding of such factors, of course, can help reduce risk and save lives. To learn more about how Droplet Digital PCR is being wielded to fight against cancer, we caught up with two researchers – Muneesh Tewari and Jason Bielas— who are using Bio-Rad’s QX100™ Droplet Digital™ PCR system in their quest to break through the current limits of nucleic acid detection and quantification.
Posts Tagged ‘droplet digital pcr’
Transforming Cancer Research: Droplet Digital™ PCR Plays Integral Role at Fred Hutchinson Cancer Research Center:: Posted by American Biotechnologist on 04-15-2014
Since its introduction two years ago, Droplet Digital PCR has transformed multiple fields of research, including infectious disease, cancer biomarker analysis, and genomic variation analysis. In this seminar, Dr. David Dodd describes the application of ddPCR in his research studies with Prof. David Corey and colleagues at UT Southwestern Medical Center at Dallas. The Corey Lab focuses on using antisense oligonucleotides and duplex RNAs to control gene expression in human cancer cells.
Clearly digital PCR has come a long way in recent years, thanks in large measure to the development of commercial systems like the QX200. These technology advances seem to indicate a tipping point where a greater number of researchers will soon have access to the technology, which will spur development of new applications that take advantage of the full capabilities of digital PCR and move scientists towards more robust biomarker studies and even single cell analyses.
Could the next revolution in PCR be digital? In an article appearing in Biotechniques, Nathan Blow takes a look at the history of digital PCR and why the methodology might have finally reached a tipping point in development.
Read the article to find out:
- What makes digital PCR different from traditional PCR
- A short history of digital PCR
- Why did it take so long for the technology to catch on with developers and researchers?
- How does digital PCR compare to real-time-quantitative PCR?
To learn more read Digital PCR: Separating from the Pack.