Posts Tagged ‘ChemiDoc’

To Film or Not to Film

 :: Posted by American Biotechnologist on 11-25-2014

The ChemiDoc Touch Imaging System is Bio-Rad’s new digital imaging solution for gel and western blot analysis. Designed for efficiency and ease of use, it integrates a touch-screen interface with Image Lab™ Touch Software to give users a fast workflow for acquiring and analyzing publication-quality image data. As a part of Bio-Rad’s V3 Western Workflow™, the ChemiDoc Touch Imaging System enables a western blotting protocol improved with multiple validation steps, streamlined completion times, and quantitative results using stain-free gel chemistry.

We gave this new imaging system to several researchers to test. Each one started the process as a user of X-ray film for western blot imaging. Some reported having tried digital imaging in the past, but not being convinced at that time to give up film. Each sat down for an interview regarding how he used the ChemiDoc Touch Imaging System and provided a representative blot image created using the instrument.

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Finding Leukemia Targets with ChemiDoc™ Touch Imaging

 :: Posted by American Biotechnologist on 11-11-2014

Better Than Film

 :: Posted by American Biotechnologist on 09-23-2014

Bio-Rad Laboratories announced the launch of its ChemiDoc Touch Imaging System. This system is a significant advance in chemiluminescent western blot detection, surpassing the performance of film and the convenience of other digital imaging systems.

Until now, digital imaging systems failed to deliver the sensitivity and resolution of film. The new ChemiDoc Touch System, however, allows detection of faint bands missed by film and produces publication-quality images.

The ChemiDoc Touch Imaging System is better able than film to detect faint bands.

The ChemiDoc Touch Imaging System is better able than film to detect faint bands.

The ChemiDoc Touch System outperforms film in other ways as well. When using film to image abundant proteins, strong bands quickly saturate and become unquantifiable. Saturated bands can also obscure the signal from adjacent faint bands, making western blotting with film challenging. The ChemiDoc Touch System addresses these issues through its wide dynamic range, which permits easy and reliable quantitation even of highly abundant proteins, and through its ability to optimize exposure for each protein of interest.

Dr. Ernesto Diaz-Flores, PhD, an assistant adjunct professor at the University of California, San Francisco (UCSF), conducted early tests on the Bio-Rad product. Dr. Diaz-Flores used the imager to simultaneously measure protein expression level changes of up to 30 different proteins in samples from leukemia patients to understand how gene mutations alter protein pathways that might represent novel therapeutic targets.

“My goal in collaborating on this project with Bio-Rad was to help develop the next generation technology required to advance protein quantification analysis and its impact in research,” said Dr. Diaz-Flores. “We found that the technology outperformed film and other imaging technologies, as it allows us to simultaneously visualize and quantitate both high and low expression proteins in a matter of seconds. It also allowed us to determine fold induction or protein reduction in high resolution and correlate these levels to drug response in multiple protein assays in a time-efficient manner.”

Capturing images with the ChemiDoc Touch System is easy. Unlike the often sluggish response of other imagers, the ChemiDoc Touch System offers a smooth, intuitive user experience that makes capturing, reviewing, selecting, and exporting images efficient and straightforward.

The imager also allows stain-free imaging, a technology exclusive to Bio-Rad. Using the stain-free enabled V3 Western Workflow™, researchers can quickly determine whether their western blot is proceeding as planned using the imager at multiple built-in checkpoints. Researchers can also use stain-free technology to perform total protein normalization for easier and more reliable protein quantitation.

To learn more about the ChemiDoc Touch System and how it provides a better user experience than film, visit www.bio-rad.com/ChemiDocTouchPR.

Chemiluminescence, Multiplex Fluorescence and Routine Gel Imaging All In One

 :: Posted by American Biotechnologist on 11-13-2012

Obtaining Picture Perfect Results

 :: Posted by American Biotechnologist on 06-07-2012

“If you don’t have accurate imaging, you’re not getting a clear picture of what’s happening.”

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