Posts Tagged ‘Cell Sorter’

Your Personal Cell Sorter

 :: Posted by American Biotechnologist on 06-26-2013

S3 Cell SorterBio-Rad Laboratories, Inc. today announced the launch of its S3 cell sorter, an automated benchtop cell sorter that even novices find easy to use.

Historically, cell sorting systems have primarily been used at core labs due to the high cost of equipment and the sophisticated methods needed by immunologists, the systems’ main users. As more researchers began to use the instruments for sorting experiments requiring less than four fluorescent parameters, wait-list times ballooned.

“Cell sorting and flow cytometry are not just tools for immunologists anymore,” said Steve Kulisch, manager of the cell biology business unit at Bio-Rad. “The S3 cell sorter meets the needs of most researchers at a very affordable price and can ease bottlenecks at core facilities by serving as a supplementary instrument for basic cell sorting work.”

The S3 cell sorter is equipped with one or two lasers (488 nm and either 561 or 640 nm) enabling up to four-color detection along with forward- and side-scatter detectors. The S3 system is ideal for sorting cells expressing fluorescent proteins and cells labeled with fluorescent markers, and for isolating individual cells for single-cell PCR or digital PCR studies. The S3 cell sorter allows researchers to sort cells at high speeds while maintaining high sensitivity and purity.

Key benefits include:

  • Simplified experimental setup — automatic instrument setup and calibration in less than 30 minutes with minimal training
  • Automatic cleaning — reduced carryover thanks to a dual-position loading stage that ensures the sample line is cleaned between sorting runs
  • Small benchtop footprint — internalized fluidics and temperature control system for a compact size (2.3 × 2.1 × 2.1 ft)

For more information about the S3 cell sorter, please visit www.bio-rad.com/cellsorter.

Bio-Rad’s S3™ Cell Sorter Chosen for Annual Course in Flow Cytometry

 :: Posted by American Biotechnologist on 06-20-2013

Bio-Rad’s S3 cell sorter will be used as a training instrument at the 36th Annual Research Course in Flow Cytometry, held at the University of New Mexico June 8–14, 2013.

Prof. David Galbraith of the University of Arizona.

“We chose the S3 cell sorter because it represents one example of an emerging trend in flow cytometric instrumentation, encompassing automated setup and operation as a sorter, a drastically reduced capital cost, and an accompanying small laboratory footprint,” said Dr. David Galbraith, co-director, with Teresa Hawley, of the Fluorescent Protein Analysis and Sorting lab session.

The research methods course will be offered with an emphasis on applications in cell biology, immunology, cell labeling, fluorescent protein analysis, bead-based assays, screening, and sorting. Distinguished members of the flow cytometry community will teach a number of laboratory sessions and lectures aimed at individuals with some experience in flow cytometry. Participants include students, postdocs, technicians, junior faculty, and company technical representatives. All attendees will have access to a wide variety of commercial and developmental instruments.

“I anticipate many applications will emerge as a result of moving flow cytometry and cell sorting into user laboratories and away from central cores,” said Galbraith. “Nevertheless, for some activities, core facilities will remain indispensible. I want attendees to be aware of these considerations, and to appreciate the capabilities of the new-generation instruments like the S3 cell sorter.”

The Annual Research Course in Flow Cytometry is the longest-running flow cytometry training course in the U.S. It alternates between Bowdoin College in Maine and the University of New Mexico at Albuquerque.

For more information about the S3 cell sorter, please visit www.bio-rad.com/cellsorter.

About the S3 Cell Sorter
The S3 cell sorter is an automated, easy-to-use, benchtop cell sorter equipped with one or two lasers, forward- and side-scatter detectors, and up to four fluorescent detectors. The S3 cell sorter and its ProSort™ software were designed to enable even novices to set up and calibrate the instrument in less than 30 minutes with minimal user input. Automated system monitoring features create a “walk-away” capability during the sort process. The simplicity of operation and the price point of the S3 cell sorter make cell sorting more accessible to new labs and core facilities that previously could not afford or support a flow cytometry instrument.

Discover a New Cell Sorting Experience

 :: Posted by American Biotechnologist on 05-06-2013

Automating and Simplifying Cell Sorting

 :: Posted by American Biotechnologist on 01-07-2013

Bio-Rad Laboratories and Propel Labs, a biotechnology instrumentation company whose principals helped to develop the MoFlo cell sorter and the CyAn ADP Analyzer, have partnered to bring researchers the new evolution in cell sorting, the S3 cell sorter. The S3 cell sorter is an automated benchtop cell sorter that contains one to two lasers and up to four fluorescence detectors, along with forward and side scatter detectors. It uses state-of-the-art ProDrop™ technology for automation of drop delay calculation and droplet break off monitoring, thereby simplifying one of the most complex aspects of cell sorting. Without compromising performance and sensitivity, this affordable cell sorter provides exceptional sort purity and yield results. This system is ideal for sorting cells expressing fluorescent proteins like GFP or cells labeled with fluorescent markers.

The S3 cell sorter offers researchers:

  • Simplified instrument setup — automated drop delay calculation and droplet break-off monitoring enable precise 1–4 color sorting with minimal training
  • Reduced carryover — dual-position loading stage ensures the sample line is clean between sort runs
  • Compact design — only 2.3 x 2.1 x 2.1 ft in dimension, it includes onboard fluidics and temperature control system
  • Intuitive interface — user-friendly ProSort™ software for effortless instrument control and sort logic definition

The S3 cell sorter makes cell sorting easy and convenient for the most common experiments. In addition, it provides expertise and experience in cell sorting from Propel Labs along with excellent technical and service support from Bio-Rad.

MoFlo and CyAn are trademarks of Beckman Coulter Inc.