Posts Tagged ‘biomarker’

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Bio-Rad Reprint: Mutational Analysis of the ATM Gene in Familial Breast Cancer Using iProof High-Fidelity DNA Polymerase

 :: Posted by American Biotechnologist on 12-30-2009

Breast Cancer is the most common cancer among women in the developed world. In 5-10% of the cases it is thought to be heritable. Two highly penetrant breast cancer genes have been identified. However, a large number of cases with familial predispositon to breast cancer cannot be explained by mutations in these two genes.

See the following article to learn how iProof polymerase significantly reduced PCR cycling time and successfully and reliably amplified sequencing quality DNA fragments that had failed repeatedly using previous methods.

Mutational Analysis of the ATM Gene using iProof

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No Comments » | Protocols

Great Tool for 2D PAGE Users

 :: Posted by American Biotechnologist on 12-25-2009

If you are actively involved in 2D experiments you must check out the 2D Doctor. It is a great tool for troubleshooting 2D experiments which can often be filled with anguish (aka streaking) and frustration (yucky gels). The tool shows you pictures of typical gels and asks you to click on the one that looks most like yours. It then gives suggestions on how to troubleshoot your experiment. If you are still stumped, there is a database of 2D-Doctor references and how to videos. And as a final (or maybe a first) resort you can click on the ask the 2D expert button to leave a detailed message for a Bio-Rad scientist who will get back to you.

Yes…2D is frustrating but it can also be very rewarding. It allows you to get tons of information from just a few experiments. Don’t despair! Help is available.

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No Comments » | Bio-Rad Tutorial, Proteomics

Are you using the right reference genes?

 :: Posted by American Biotechnologist on 12-10-2009

Considering Real-Time PCR for gene expression analysis. Have you tested multiple reference genes or are you just going to run with your favorite such ast 18S? Check out this important article before you move forward. It could save you lots of grief in the long run.

Suitable reference genes for real-time PCR in human HBV-related hepatocellular carcinoma with different clinical prognoses
Li-Yun Fu et al. BMC Cancer 2009, 9:49

Housekeeping genes are routinely used as endogenous references to account for experimental differences in gene expression assays. However, recent reports show that they could be de-regulated in different diseases, model animals, or even under varied experimental conditions, which may lead to unreliable results and consequently misinterpretations. This study focused on the selection of suitable reference genes for quantitative PCR in human hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) with different clinical outcomes.

Suitable Reference Gene Selection 2009

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No Comments » | Protocols

Lucid Proteomics System

 :: Posted by American Biotechnologist on 12-07-2009

Once upon a Proteomics Study… enjoy this fanciful look at how combining top-down and bottom-up proteomics will change biomarker research forever.

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No Comments » | cool tools, Videos

Phase 2 of 2D Gel Reproducibility Effort Yields ‘Significant’ Confidence Levels

 :: Posted by American Biotechnologist on 10-13-2009

New story today published in Genomeweb shows that 2D Gel Electrophoresis is once again gaining momentum in the world of proteomics and biomarker research.

In the second phase of a campaign to test the reproducibility of 2D gel technology, 10 out of 17 laboratories, or 60 percent, were able to generate gel images that fell within a 95-percent confidence level in an inter-laboratory study.

As a result, the methods developed in both phases of the campaign, called Fixing Proteomics, now serve as standards by which proteomics researchers using 2D gels can benchmark themselves, according to the campaign’s organizers.

For more on the story see http://www.genomeweb.com/proteomics/phase-2-2d-gel-reproducibility-effort-yields-significant-confidence-levels

Avi

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No Comments » | Proteomics
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