Posts Tagged ‘Bio-Rad’

Seminar on New Standards in Real-Time PCR

 :: Posted by American Biotechnologist on 10-27-2009

There has been a lot of buzz lately on the newly published guidelines detailing the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines published by Bustin et al. in Clinical Chemistry 55:4, 2009. The paper was coauthored by several important PCR authorities including Jo Vandesompele and is therefore sure to become an important standard for real-time PCR publications. Dr. Sean Taylor of Bio-Rad has put together several short youtube videos explaining the importance of MIQE and how it can be applied to the bench.

Here are the links:

Part 1 of 4

Part 2 of 4

Part 3 of 4

Part 4 of 4

Real-Time PCR Applications Guide

 :: Posted by American Biotechnologist on 10-16-2009

Nucleic acid amplification and detection are among the most valuable techniques used in biological research today. Scientists in all areas of research — basic science, biotechnology, medicine, forensic science, diagnostics, and more — rely on these methods for a wide range of applications. For some applications,
qualitative nucleic acid detection is sufficient. Other applications, however, demand a quantitative analysis. Choosing the best method for your application requires a broad knowledge of available technology.

This guide from Bio-Rad provides an introduction to many of the technical aspects of real-time PCR. It includes guidelines for designing the best real-time PCR assay for your experiments and explains how real-time PCR data are used in various applications.

To download the Guide click here (it will take a moment): Real-Time PCR Applications Guide