Posts Tagged ‘Bio-Rad’

High Resolution Melt Analysis Applicatons

 :: Posted by American Biotechnologist on 12-29-2009

Genomics is rapidly expanding beyond merely identifying the genetic makeup of organisms to encompass studies of how variations in genotype impact physiological functions. See the article below which was published in the October 15th edition of Genetic Engineering and Biotechnology news to learn more about high-res met analysis as an alternative to probe-based genotyping.
High-Resolution Melt Analysis Applications

Great Tool for 2D PAGE Users

 :: Posted by American Biotechnologist on 12-25-2009

If you are actively involved in 2D experiments you must check out the 2D Doctor. It is a great tool for troubleshooting 2D experiments which can often be filled with anguish (aka streaking) and frustration (yucky gels). The tool shows you pictures of typical gels and asks you to click on the one that looks most like yours. It then gives suggestions on how to troubleshoot your experiment. If you are still stumped, there is a database of 2D-Doctor references and how to videos. And as a final (or maybe a first) resort you can click on the ask the 2D expert button to leave a detailed message for a Bio-Rad scientist who will get back to you.

Yes…2D is frustrating but it can also be very rewarding. It allows you to get tons of information from just a few experiments. Don’t despair! Help is available.

Bio-Rad Tutorial:Troubleshooting Electroporation

 :: Posted by American Biotechnologist on 12-21-2009

Bio-Rad Laboratories has been at the forefront of gene transfer technology for decades. The Bio-Rad Gene Pulser is synonymous with electroporation and there are hundreds (thousands?) of publications that give credit to this formidable machine. In spite of its longevity, the Bio-Rad Gene Pulser X-cell continues to be a favorite tool of cell biologists worldwide. Click here to view a flash presentation describing the system and what it does including how to set up and troubleshoot electroporation experiments.

xcell tile

Bio-Rad Tutorial: How to avoid streaking in 2D Gels

 :: Posted by American Biotechnologist on 11-30-2009

2D Gel Electrophoresis has become an important technique for identifying proteins and evaluating their expression levels and functionality in proteomics experiments. Due to the complex nature of working with an alphabet soup of proteins, there are many factors that need to be considered when performing this technique. It is critical to pay attention to your sample preparation if you wish to obtain good results. In the latest Bio-Rad Tutorial, you will learn how to effectivley prepare samples for 2DGE and how to optimize your focusing conditions to avoid dreaded gel streaks.

avoiding streaking on 2dge

Real-Time Quantitative PCR Data Analysis Tutorial

 :: Posted by American Biotechnologist on 11-12-2009

Bio-Rad Laboratories has put together a great tutorial on QPCR analysis including a thorough review of what the graphs mean and an overview of Absolute and Relative methods of quantification. The tutorial also includes a great explanation of the differences between Livak, delta CT and the Pfaffl methods of analyzing your results.

Watch part II: normalization against a reference gene