Posts Tagged ‘Bio-Rad Laboratories’

Life Since the Double Helix

 :: Posted by American Biotechnologist on 02-18-2014

The discovery of the double-helix structure of DNA 60 years ago led to a revolution in biological science, opening the floodgates for myriad subsequent discoveries and spawning new fields of research. Bio-Rad has been there from the beginning, helping scientists, educators, and clinicians advance basic research and improve healthcare. As we celebrate Bio-Rad’s diamond anniversary, we reflect on the major events in the evolution of life science research, from biochemistry to molecular biology and beyond, and the emergence of modern biotechnology.

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Purifying Untagged Protein With Ease

 :: Posted by American Biotechnologist on 02-10-2014

High purity protein is a common requirement for biochemical and structural studies. A common approach is to recombinantly express an affinity-tagged version of the protein of interest. This is, however, not always a viable option. Some proteins are unstable or inactive once tagged or require posttranslational modifications that do not permit recombinant expression. In these cases, researchers often settle for lower purity protein rather than exhaustively explore purification options, since the purification optimization process can be time and labor intensive when no particular column resins or buffer conditions are dictated by an affinity tag.

Bio-Rad Laboratories has recently released a study demonstrating how to purify untagged protein to high homogeneity without undergoing laborious manual troubleshooting steps. Bio-Rad’s ChromLab software can be programmed to execute several runs sequentially thereby automating and accelerating this tedious process.

To learn how to purify untagged protein with ease read Protein Purification Workflow Development Using Bio-Rad’s NGC™ Chromatography System.

Droplet Digital™ PCR: Guidelines for Multiplexing

 :: Posted by American Biotechnologist on 01-30-2014

Droplet Digital PCR (ddPCR™) enables accurate, precise, and sensitive quantification of specific nucleic acid sequences. In addition to the standard detection of two targets using two different fluorophores, it is possible to increase the number of targets detected by varying parameters that affect PCR efficiency and end-point fluorescence. In this case, we describe a method to multiplex assays by varying the concentrations of primers and probes or the type of fluorophores used. This allows users to expand the number of simultaneously detected targets up to four. Increasing the number of potential targets per test is a significant improvement for ddPCR, dramatically augmenting the information output of each sample.

For more information download the Droplet Digital™ PCR: Guidelines for Multiplexing from Bio-Rad Laboratories.

Investigating Copy Number Variation in Induced Pluripotent Stem Cells

 :: Posted by American Biotechnologist on 01-29-2014

Using Droplet Digital™ PCR to Diagnose Leukemia

 :: Posted by American Biotechnologist on 01-20-2014