Posts Tagged ‘Bio-Rad Laboratories’

Staining of Live Cells for Multicolor Flow Cytometry Experiments

 :: Posted by American Biotechnologist on 05-07-2014

Cytotrack_productsBio-Rad Laboratories, Inc. today announced the launch of its CytoTrack Cell Proliferation Assays that provide unmatched flexibility when designing a multicolor flow cytometry experiment, allowing researchers to efficiently stain and track live cells in four colors for up to 10 generations — two more generations than other currently available methods can follow.

CFDA-SE, the most commonly used reagent for monitoring cell proliferation, is difficult to use in multicolor experiments because it has the same spectra as green fluorescent protein and the most frequently used fluorophore, fluorescein.

Unlike CFDA-SE and other popular cell proliferation assays that are available in only one color, Bio-Rad’s CytoTrack Assays are available in four different emission wavelengths ranging from blue to far red. This allows researchers to choose the one most suitable for their multicolor flow cytometric experiment.

The CytoTrack Assay dye reacts with primary amines using a proprietary chemistry that provides effective labeling of cells without the large efflux usually seen with CFDA-SE.

“This unique feature allows researchers to easily visualize up to 10 cell divisions, making it ideal for those interested in studying the induction and inhibition of cell division in any in vitro experimental model,” said Mary Thao, Bio-Rad Product Manager in the Gene Expression Division.

CytoTrack Assays come in easy-to-use vials with material sufficient for 50 experiments. The dye can be added directly into the culture medium, allowing researchers to save time.

Bio-Rad offers additional reagents and consumables to complete each step of flow cytometry experiments, such as cell viability assays, primary antibodies, and antibody labeling kits.

For more information about Bio-Rad’s cell proliferation assays, please visit bio-rad.com/cellproliferation

Bio-Rad Laboratories Awarded Best New Life Sciences Product of 2013

 :: Posted by American Biotechnologist on 05-01-2014

SelectScience was delighted to announce the QX200™ Droplet Digital™ PCR System, by Bio-Rad, as the winner of the Scientists’ Choice Award for Best New Life Sciences Product of 2013. The award was presented to Bio-Rad at the American Association for Cancer Research (AACR) Annual Meeting 2014.

Click on the photo below to navigate to the SelectScience website and view the video.

select science ddpcr award

2-D Electrophoresis Tips & Tricks – Part II

 :: Posted by American Biotechnologist on 04-28-2014

In part I of the 2-D electrophoresis series (2DGE), Bio-Rad Laboratories’ Anton Posch talked about proper sample preparation techniques for good 2DGE. In this second and final part of the series, Dr. Posch covers IEF separation, using immobilized pH gradient (IPG) strips, running second-dimension PAGE, spot detection, gel matching, and data analysis. If you want to perform great 2DGE, it is well worth your while to watch these two tutorials.

2-D Electrophoresis Tips & Tricks – Part I

 :: Posted by American Biotechnologist on 04-22-2014

Transforming Cancer Research: Droplet Digital™ PCR Plays Integral Role at Fred Hutchinson Cancer Research Center

 :: Posted by American Biotechnologist on 04-15-2014

It’s no surprise that Droplet Digital PCR (ddPCR) has found its place in the world of cancer research. Bio-Rad’s ddPCR™ technology has a remarkable ability to quantify miniscule amounts of target DNA and RNA, and thus can contribute to early detection of rare tumorigenic mutations against a high background of “normal” DNA as well as to other applications, including identifying cancer subtypes, optimizing drug treatment plans, and studying tumor evolution. At the Fred Hutchinson Cancer Research Center in Seattle, scientists are producing some of the most important advances in prevention, early detection and treatment of cancer and other diseases. They are studying the disease process from every angle seeking to uncover factors that influence a person’s likelihood of getting cancer. Understanding of such factors, of course, can help reduce risk and save lives. To learn more about how Droplet Digital PCR is being wielded to fight against cancer, we caught up with two researchers – Muneesh Tewari and Jason Bielas— who are using Bio-Rad’s QX100™ Droplet Digital™ PCR system in their quest to break through the current limits of nucleic acid detection and quantification.

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