:: Posted by American Biotechnologist on 12-25-2009
If you are actively involved in 2D experiments you must check out the 2D Doctor. It is a great tool for troubleshooting 2D experiments which can often be filled with anguish (aka streaking) and frustration (yucky gels). The tool shows you pictures of typical gels and asks you to click on the one that looks most like yours. It then gives suggestions on how to troubleshoot your experiment. If you are still stumped, there is a database of 2D-Doctor references and how to videos. And as a final (or maybe a first) resort you can click on the ask the 2D expert button to leave a detailed message for a Bio-Rad scientist who will get back to you.
Yes…2D is frustrating but it can also be very rewarding. It allows you to get tons of information from just a few experiments. Don’t despair! Help is available.
:: Posted by American Biotechnologist on 11-30-2009
2D Gel Electrophoresis has become an important technique for identifying proteins and evaluating their expression levels and functionality in proteomics experiments. Due to the complex nature of working with an alphabet soup of proteins, there are many factors that need to be considered when performing this technique. It is critical to pay attention to your sample preparation if you wish to obtain good results. In the latest Bio-Rad Tutorial, you will learn how to effectivley prepare samples for 2DGE and how to optimize your focusing conditions to avoid dreaded gel streaks.
:: Posted by American Biotechnologist on 10-13-2009
New story today published in Genomeweb shows that 2D Gel Electrophoresis is once again gaining momentum in the world of proteomics and biomarker research.
In the second phase of a campaign to test the reproducibility of 2D gel technology, 10 out of 17 laboratories, or 60 percent, were able to generate gel images that fell within a 95-percent confidence level in an inter-laboratory study.
As a result, the methods developed in both phases of the campaign, called Fixing Proteomics, now serve as standards by which proteomics researchers using 2D gels can benchmark themselves, according to the campaign’s organizers.
For more on the story see http://www.genomeweb.com/proteomics/phase-2-2d-gel-reproducibility-effort-yields-significant-confidence-levels