Posts Tagged ‘2D Gel Electrophoresis’

Improving the statistic significe of your Western Blots

 :: Posted by American Biotechnologist on 09-08-2011

Western Blotting is probably one of the most ubiquitous techniques in the molecular biology lab and relatively easy to perform. Yet many of us have been frustrated with statistically insignificant results or protein bands that appear either too dark or too light to quantitate.

Well, do not despair! There are many things you can do to help improve the quality of your blots and increase your likelihood of obtaining statistically significant results!

In the video below, you will learn about the many factors affecting western blot analysis (such as detection limit and dynamic range limitations of film and overloaded gels) and what can be done to improve your chances of success.

The presentation was given by Bio-Rad Laboratories Field Application Specialist Dr. Sean Taylor as part of an intimate customer training. Some of the references in the presentation may be specific for that particular customer but the general information contained in this presentation is highly valuable to all molecular biology labs.

Still stuck on tank or semi-dry protein blotting?

 :: Posted by American Biotechnologist on 08-17-2011

Learn why the Trans Blot Turbo is faster than tank and semi-dry blotting techniques.

Bio-Rad Video 5/11/11 from Lab Manager Magazine on Vimeo.

Quality controlling 2D gel data and why you should do it

 :: Posted by American Biotechnologist on 05-31-2010

Be sure to check out the upcoming webinar on Wednesday June 9th from our friends at Ludesi.

Learn how to use the free Gel IQ tool to measure the correctness of your image analysis output.

* Introducing you to Combined Correctness: a generic metric for calculating a quality score
* Using the free software tool “Gel IQ” to calculate a quality score on your 2D gel image analysis data
* How to import your analysis project into Gel IQ
* Applying the correct evaluation settings
* Evaluating spot detection and matching correctness
* Interpreting your Combined Correctness score
* Using Gel IQ to quality control and optimize your 2D gel analysis protocols

Wednesday, June 9, 2010
12 PM EDT / 9 AM PDT (US) / 6 PM CET (Europe)
Click here to register for the Wednesday time slot

Proteomics Refined

 :: Posted by American Biotechnologist on 05-21-2010

As a proteomics fan in general and a 2D Gel Electrophoresis fan specifically, I have been following the Ludesi Blog which I have found to be a resource rich in 2D information including a knowledgebase, webinars and an great tool that pulls together all proteomics related tweets from twitter. For those of you who are not familiar with Ludesi, they are a Swedish company that was founded in 2001 by Ola Forsstrom-Olsson who was listed in MIT Technology Review’s Top 100 list of the World’s Best Technology Innovators Under 35 in 2004.

In addition to having a great blog, Ludesi produces fantastic 2D gel imaging software and provides an analytical service that is fast, user friendly, compatible with all types of stains, has great technical support and a unique pay as you go system. But the best of all is that the experts at Ludesi analyze the gels for you and save you some serious computer time!

To get started:

– Download REDFIN software at www.expressionproteomics.com/redfin and follow the registration
procedure
– Upload 2-D gel images and purchase credits.
– Each gel is analyzed by multiple professionals at Ludesi.
– Data and gels are stored on the redfin site and can be accessed via the secure server from any computer (anywhere) at any time.
– Since Redfin is a Software As A Service (SAAS) it is automatically updated every time you log in (no need to pay for updates
– Best of all…you only PAY FOR WHAT YOU USE!

For a limited time, Bio-Rad has arranged for new customers to receive three free credits when first trying out the service.

What does that mean? Well, to have a gel analyzed using the basic service (appropriate for almost all researchers) you will need 2 credits and to analyze a gel using the Pro service (removes streaks, and artifacts and expands upon quality assurance for spot detection and matching) you will require 10 credits per gel.

To get your free credits, download redfin from www.expressionproteomics.com/redfin, click on the “use value code” button and enter “TRYREDFIN” and away you go!

To read more about Redfin, see the a href=’http://www.americanbiotechnologist.com/blog/wp-content/uploads/2010/05/Redfin-brochure.pdf’>Redfin brochure

For a thorough review on the limits, benefits, and perspectives of gel-based proteomic approaches see the recently published review article in Proteome Science.

Chevalier, F. (2010). Highlights on the capacities of “Gel-based” proteomics Proteome Science, 8 (1) DOI: 10.1186/1477-5956-8-23

A gel worthy of high-impact publication

 :: Posted by American Biotechnologist on 03-02-2010

Yes…SDS PAGE is boring. But it’s an indispensable part of our bench research. We put tons of time into designing experiments, sample prep and playing video games (oops…Freudian slip). It is therefore absolute torture when we load our precious sample into a gel, run it for 30 to 60 minutes only to discover that the quality of the gel has significantly compromised what promised to be an award winning figure worthy of publication in the likes of Nature or Science (would you even think of publishing anywhere else?).

For as long as I can remember, Bio-Rad has been at the forefront of electrophoresis technology. There is hardly a graduate student out there who has not come across a green MP3 or MP tetra electrophoresis unit during the course of their graduate work. Now Bio-Rad has released a new SDS Polyacrylamide Gel that provides high resolution separation regardless of the origin or complexity of the sample. Moreover, whether you are a 2-D researcher, a “stainer” or a run of the mill 1D separation type of scientist, the new TGX gels will help you produce results that your reviewers will be proud of! See the tech note for more details.