Fast qPCR assay optimization and validation techniques for HTS

Here’s a a great powerpoint presentation by Frank Bizouarn, International Field Application Specialist, Bio-Rad Laboratories, on how to perform fast qPCR for high throughput screening.

The presentation goes through the intricacies of primer design, ideal amplicon characteristics, troubleshooting with a thermal gradient, how reagents can impact your experiment, primer titration, assay sensitivity, standard curves, the affect of PCR inhibitors, how scale affects reproducibility, throughput and speed.

Download the slides by clicking here or for other great presentations visit www.bio-rad.com/genomics/pcrsupport

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