Bio-Rad Laboratories, Inc. today announced the launch of its PrimePCR probe assays for quantitative real-time PCR (qPCR). The new assays for human and mouse genomes set the bar for specificity, sensitivity, and linear dynamic range.
Bio-Rad has again partnered with Biogazelle, a qPCR data analysis and services company, to design probe assays for qPCR gene expression analysis. The fluorescently labeled oligonucleotide probes were designed to complement Bio-Rad’s high-specificity PCR primer assays, enabling multiplex assays and eliminating the need for melt curve analysis.
The primer pair of each probe assay has been wet-lab validated following strict assay performance standards for amplification efficiency, specificity, sensitivity, and linear dynamic range. These specifications help researchers meet the MIQE (minimum information for publication of quantitative real-time PCR experiments) guidelines.
The assays are available with probes labeled with FAM, HEX, or TEX 615 fluorophores, and each includes a gene-specific synthetic qPCR template designed as a positive control. The probe assays can be used with CFX real-time PCR detection systems and data analysis software, streamlining PCR data collection and analysis.
PrimePCR probe assays are available in 500, 1,000, and 2,500 reaction sizes.
For more information on Bio-Rad’s PrimePCR products, please visit www.bio-rad.com/PrimePCR.