If you are actively involved in 2D experiments you must check out the 2D Doctor. It is a great tool for troubleshooting 2D experiments which can often be filled with anguish (aka streaking) and frustration (yucky gels). The tool shows you pictures of typical gels and asks you to click on the one that looks most like yours. It then gives suggestions on how to troubleshoot your experiment. If you are still stumped, there is a database of 2D-Doctor references and how to videos. And as a final (or maybe a first) resort you can click on the ask the 2D expert button to leave a detailed message for a Bio-Rad scientist who will get back to you.
Yes…2D is frustrating but it can also be very rewarding. It allows you to get tons of information from just a few experiments. Don’t despair! Help is available.