Enteroviral Replication Revealed

Enteroviruses such as Poliovirus and Hepatitis are notorious for the disastrous effects they cause upon infection. A study recent published in Cell contributes a significant finding to the field of RNA virus replication. The Altan-Bonnet lab at Rutgers University has shown how that upon infection, RNA viruses generate specialized RNA replication organelles enriched in phosphatidylinositol-4-phosphate (PI4P) lipids and that drug therapy is able to block the activity of the cellular enzyme once it has been activated. You can see the press release on Lab Spaces or the Altan-Bonnet lab video below.

To summarize the findings in a nutshell: Viral RNA replication takes place on the host cell’s intracellular membrane which is facilitated by the 3A membrane protein. 3A is inserted in to the host membrane, recruits PI4P which then selectively binds the RNA polymerase to facilitate replication. The video also shows replication of the virus using a GFP tagged that binds the PI4P lipid. As the infection proceeds the video demonstrated increased GFP expression. (You gotta love real time video presentations as part of a quality paper).

Hsu, N., Ilnytska, O., Belov, G., Santiana, M., Chen, Y., Takvorian, P., Pau, C., van der Schaar, H., Kaushik-Basu, N., & Balla, T. (2010). Viral Reorganization of the Secretory Pathway Generates Distinct Organelles for RNA Replication Cell, 141 (5), 799-811 DOI: 10.1016/j.cell.2010.03.050

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