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:: Posted by American Biotechnologist on 05-26-2010
In this latest tutorial by the Bio-Rad Technical Support Team you will learn how to validate a SYBR Green I assay for real-time quantitative PCR (qPCR). It is important to validate all qPCR assays prior to commencing your experiment in order to confirm that your reactions will be efficient, specific, sensitive and reproducible. SYBR Green I assay validation should also be done when using a new set of primers or a new DNA template/primer combination regardless of the chemistry that you intend to use in your actual experiment.
Hats off to Linda Lingelbach and Kathy Silvey of Bio-Rad Technical Support who worked hard at putting this enlightening video together.
:: Posted by American Biotechnologist on 05-24-2010
As scientists we are “naturally obsessed.” We have a need to find the answer and can’t stop working until we get it. So how do we find the time to watch this one hour dramatic story by Richard and Carole Rifkind about life in a molecular biology lab? Although there are many health related stories in the media (1), it’s not every day that you get to see “our” story on television. Besides, what else are we going to do with ourselves during our next “one hour incubation time?”
(1) Wang, Z., & Gantz, W. (2010). Health Content in Local Television News: A Current Appraisal Health Communication, 25 (3), 230-237 DOI: 10.1080/10410231003698903
:: Posted by American Biotechnologist on 05-12-2010
Bio-Rad’s latest gadget is the TC10 automated cell counter, which accurately and reproducibly provides total mammalian cell counts in 30 seconds or less—eliminating the need for manually counting cells under the microscope using a hemocytometer.
The TC10 cell counter completes the counting of mammalian cells—suspended or re-suspended adherent—in one simple step, initiating a count immediately upon slide insertion. Unlike other cell counting devices, which may draw inaccurate conclusions from using a single plane of focus to assess cell viability, the TC10 cell counter scores each sample across multiple planes. The unique auto-focus technology and image analysis algorithm provide accurate and reproducible cell counts in less than 30 seconds for cell concentrations between 5 x 104 and 1 x 107 cells/ml and cells within a 6–50 µm diameter range. Since only 10 µl of suspended cells is required for counting, precious sample is conserved.
Cell viability can also be assessed with the TC10 cell counter with the use of trypan blue, a dye which is excluded from live cells and incorporated into dead cells. The system recognizes the presence of trypan blue without user intervention and provides both cell count and cell viability, in less than 30 seconds.
The TC10 cell counter saves time with its on-board dilution calculator which can determine the volume adjustments needed to achieve the cell concentration for the next passage or experiment. In experiments using trypan blue, the dilution calculation is based on the number of live cells.
Using the USB port, researchers can export annotated JPEG image files of their samples for further analysis or download counting data in a spreadsheet compatible format. Results can also be printed directly onto labels using the separate TC10 printer.
For an online demo of how to use the TC10 and other video tutorials including the TC10’s use in gene expression studies, a cell biology primer and a basic cell culture introductory document visit the TC10 website.
:: Posted by American Biotechnologist on 05-05-2010
Sometimes working at the bench can be tedious and frustrating. Preparing samples, running experiments, analyzing data, REDOING experiments (ugh!)… And what is all the hard work for? To “further science?” To be rich and famous? Here’s a video that we’ve discovered that will put a tear in your eye and pride in your heart. Way to go biotechnologists!
:: Posted by American Biotechnologist on 04-29-2010
Here’s an inspiring story told first-hand from Guido D. Nez-Mujica who presented a Google Tech Talk on Science, Innovation and entrepreneurship in developing countries. Dr. Nez-Mujica remained in his native home Venezuala instead of going abroad, to do some cheap biotechnology that is useful to people, solves unmet needs and allows us to use our knowledge for more than academic purposes.