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:: Posted by American Biotechnologist on 06-15-2010
Here’s the latest video from Bio-Rad Laboratories demonstrating how to use the Profinia Protein Purification System. The Profinia offers fast, automated affinity purification that is ready to roll with the touch of a button. Best of all, dialysis is performed on-column and you end up with desalted, purified protein with an estimated protein concentration.
Watch the video for a detailed explanation to see how it’s done.
:: Posted by American Biotechnologist on 06-02-2010
Transfecting cells can be a difficult task for even the most seasoned of cell biologists. Sure there are easy-to-transfect cell lines such as A10 Smooth Muscle Cells, COS Cells or p19 cells, but what about those pesky, hard to isolate primary cells? Or better yet, what about cells embedded in living tissue on animals or plants?! Sometime the task at hand drives you so crazy that you just want to shoot something.
Turns out that might not be such a bad idea after all. Bio-Rad’s Helios Gene Gun is a common tool for biolistic transfection of cells embeded in animal or plant tissue. The video posted below was published in the Journal of Visualized Experimentation and it will give you a great visual demonstration of the Gene Gun at work.
Woods, G., & Zito, K. (2008). Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture Journal of Visualized Experiments (12) DOI: 10.3791/675
:: Posted by American Biotechnologist on 05-26-2010
In this latest tutorial by the Bio-Rad Technical Support Team you will learn how to validate a SYBR Green I assay for real-time quantitative PCR (qPCR). It is important to validate all qPCR assays prior to commencing your experiment in order to confirm that your reactions will be efficient, specific, sensitive and reproducible. SYBR Green I assay validation should also be done when using a new set of primers or a new DNA template/primer combination regardless of the chemistry that you intend to use in your actual experiment.
Hats off to Linda Lingelbach and Kathy Silvey of Bio-Rad Technical Support who worked hard at putting this enlightening video together.
:: Posted by American Biotechnologist on 05-24-2010
As scientists we are “naturally obsessed.” We have a need to find the answer and can’t stop working until we get it. So how do we find the time to watch this one hour dramatic story by Richard and Carole Rifkind about life in a molecular biology lab? Although there are many health related stories in the media (1), it’s not every day that you get to see “our” story on television. Besides, what else are we going to do with ourselves during our next “one hour incubation time?”
(1) Wang, Z., & Gantz, W. (2010). Health Content in Local Television News: A Current Appraisal Health Communication, 25 (3), 230-237 DOI: 10.1080/10410231003698903