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:: Posted by American Biotechnologist on 11-18-2010
I’ve spoken about one of my favorite blogs in the past and I will tell you about them once again (because they continue to provide their readers with great material). Benchfly is a fun resource for lab-rats everywhere. On the site you will find both fun and instructional videos, interesting articles and tons of useful resources.
Several months ago we shared with you a video posted on Benchfly which demonstrated the difficulties we face in pronouncing foreign scientific terms. (See “the things we say” to view that post). The same group recently uploaded a protocol video describing how to save time and money when when cloning by making your own rapid ligation kit. If you are doing any cloning in your lab, I highly recommend watching the video below.
:: Posted by American Biotechnologist on 10-12-2010
I just saw this video on the Popsci website and thought it would be nice eye candy for the American Biotechnologist readership. To recap, Dr. Laura Niklason’s lab at Yale University stripped down a lung to its basic infrastructure, then repopulate it with fresh, lab-grown cells. The research first appeared in a 1999 Science paper, in another Science article this past summer and was written up (along with advances in other tissue engineering studies) in a Wall Street Journal article on June 25th. I’m not sure how Popsci determined to write about it once again this past week, but the video sure is cool and worth sharing!
If you have trouble playing the video, click here.
An un-narrated version (but a bit more detailed) can be found on youtube courtesy of Labspaces (and Yale University).
I definitely recommend checking out the labspaces post. Their posts are usually interesting and pretty well written.
:: Posted by American Biotechnologist on 09-27-2010
This video was created by XVIVO, a scientific animation company near Hartford, CT in July 2006 for Harvard biology students. The 8 minute animation took 14 months to create and has been viewed tens of thousands of times by molecular biology aficionados all across the globe. If you haven’t yet seen it, it’s well worth your time.
:: Posted by American Biotechnologist on 07-27-2010
Real Time Quantitative PCR has become a staple of almost every molecular biology lab in the world. In fact, I doubt that if asked, many scientists would be willing to admit that they don’t do real time quantitative PCR. The cost of attaining a Real Time qPCR instrument and the per assay costs have come down exponentially putting real time quantitative PCR within the reach of most molecular biology labs.
In this video tutorial by Bio-Rad Laboratories (which is applicable to ALL real time quantitative PCR platforms), you will learn the basics of Real Time qPCR and the science behind absolute and relative quantification methods. Absolute quantification is used when looking to answer the question of “how many” or “how much.” Examples include how many copies of gene x are in my sample or how much viral load is there in a given biological sample. Relative quantification, (the more popular technique for data analysis), is used when looking to measure changes or differences in gene expression between various samples. Examples include looking for upregulation or downregulation of a gene in a normal versus disease state or changes in gene expression following an siRNA gene silencing experiment.
Several methods have been published for conducting real time qPCR data analysis using relative quantification methods including Livak, delta CT and the Pfaffl methodology. The video will also explain the differences between each of these techniques and a review of their uses and limitations.
If you are very ambitious, visit the original Bio-Rad video link for a clearer picture that combines both of these youtube videos into one (youtube doesn’t allow for videos that are over 10 minutes long and tends to make text a bit blurry).
:: Posted by American Biotechnologist on 07-14-2010
The folks at The Scientist are running a contest for readers to vote for their favorite videos and websites. Browsing through the videos on The Scientist Labbies, Readers’ Choice site (be sure to watch the videos and vote for your favorite) I came across a visually stunning animation by the US Department of Energy Joint Genome Institute (DOE JGI).
The DOE JGI unites the expertise of five national laboratories to advance genomics in support of the DOE missions related to clean energy generation and environmental characterization and cleanup. JGI’s Walnut Creek, California, facility provides integrated high-throughput sequencing and computational analysis that enable systems-based scientific approaches to these challenges.
Scientists affiliated with JGI are prolific publishers of scientific articles and industrious contributors to Genbank.
Check out this mesmerizing video (the graphics are great thanks to recent graduates and staff at the Ex’pression College for Digital Arts) to learn more about JGI’s mission.