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Archive for the ‘Videos’ Category

A Video Tutorial for High Resolution Melt Analysis

 :: Posted by American Biotechnologist on 01-10-2011

High resolution melt (HRM) analysis is a relatively new technique used in detecting small variations in DNA sequences between varying populations. Important applications of HRM include SNP analysis, genotyping and methylation analysis.

In the following 20 minute tutorial presented by Sean Taylor, Field Application Specialist, Bio-Rad Laboratories, you will learn the basics of high resolution melt analysis and how to practically use it in your research. The video contains information on:

  • what is high resolution melt analysis, how does it work and why it is useful
  • various applications for high resolution melt analysis including single nucleotide polymorphism (SNP) analysis and DNA methylation analysis
  • the difference between a standard melt curved performed in routine QPCR and HRM
  • the difference between non-saturation dyes such as SYBR green and saturation dyes such as evagreen
  • the definition of the four class types of SNPS
  • critical factors for good experimental design
  • what technique you should be using for your SNP analysis (it is not one size fits all…think HRM VS Sequenome VS multiplex array
  • and more!!!

For more information on HRM see A Practical Guide to High Resolution Melt Analysis Genotyping

Real Time Quantitative PCR Software Tutorial

 :: Posted by American Biotechnologist on 01-04-2011

If you are a user or potential users of Bio-Rad’s CFX or MiniOpticon systems be sure to see the message below and the accompanying real time quantitative PCR software tutorial from Bio-Rad’s Sean Taylor, Field Applications Specialist.

I am pleased to provide our recently upgraded version of CFX Manager. Version 2.0 has significant improvements and new tools from the last version 1.6 of the software. I recently did a little test drive and have recorded a 25-minute training video to help you navigate around the new features. Some key highlights include:

1. Scheduling module so that you can schedule your time on the instrument. A great tool for a multi-user instrument.
2. Master Mix Calculator…Just type in your primer concentrations and you get the volumes required for your experiment. No more thinking and more importantly…no more mistakes in volume conversions!
3. Copy and paste wells in the plate editor. I am personally very pleased with this feature.
4. Much easier access to the data you need and very nice and new data view and data export features.

The link to the video is here:
video link

The specifications for the software can be found here:
software specifications

To access the upgrade, you can go to:
upgrade

Click on “Downloads” and you will find:

A. The CFX96 and CFX384 Real-Time PCR Detection Systems Firmware Update.

B. The upgrade patch for CFX Manager 2.0.

C. Precision Melt Analysis™ v1.1 Software Upgrade (This is only required for those who are using Version 1.0 of Precision Melt Analysis Software).

Important: The firmware update comes with detailed instructions that are easy to follow but do not hesitate to call tech support (1-800-4Bio-Rad) if you have any questions about the procedure.

I would like to take this opportunity to wish you and your families health and happiness in 2011!

Sean Taylor
Field Application Specialist
Bio-Rad Laboratories

Click here for other technical resources from Sean including his video entitled “A Practical Approach to MIQE for the Bench Scientist,” A Practical Guide to High Resolution Melt Analysis Genotyping, and the article A Simple Solution to Chromatography for High-Purity Protein Preparations: The Modular Approach

Rapid ligation the Benchfly way

 :: Posted by American Biotechnologist on 11-18-2010

I’ve spoken about one of my favorite blogs in the past and I will tell you about them once again (because they continue to provide their readers with great material). Benchfly is a fun resource for lab-rats everywhere. On the site you will find both fun and instructional videos, interesting articles and tons of useful resources.

Several months ago we shared with you a video posted on Benchfly which demonstrated the difficulties we face in pronouncing foreign scientific terms. (See “the things we say” to view that post). The same group recently uploaded a protocol video describing how to save time and money when when cloning by making your own rapid ligation kit. If you are doing any cloning in your lab, I highly recommend watching the video below.

Cool Video of Tissue Engineered Lung

 :: Posted by American Biotechnologist on 10-12-2010

I just saw this video on the Popsci website and thought it would be nice eye candy for the American Biotechnologist readership. To recap, Dr. Laura Niklason’s lab at Yale University stripped down a lung to its basic infrastructure, then repopulate it with fresh, lab-grown cells. The research first appeared in a 1999 Science paper, in another Science article this past summer and was written up (along with advances in other tissue engineering studies) in a Wall Street Journal article on June 25th. I’m not sure how Popsci determined to write about it once again this past week, but the video sure is cool and worth sharing!

If you have trouble playing the video, click here.

An un-narrated version (but a bit more detailed) can be found on youtube courtesy of Labspaces (and Yale University).

I definitely recommend checking out the labspaces post. Their posts are usually interesting and pretty well written.

A Molecular Biology Video Classic

 :: Posted by American Biotechnologist on 09-27-2010

This video was created by XVIVO, a scientific animation company near Hartford, CT in July 2006 for Harvard biology students. The 8 minute animation took 14 months to create and has been viewed tens of thousands of times by molecular biology aficionados all across the globe. If you haven’t yet seen it, it’s well worth your time.