High resolution melt (HRM) analysis is a relatively new technique used in detecting small variations in DNA sequences between varying populations. Important applications of HRM include SNP analysis, genotyping and methylation analysis.
In the following 20 minute tutorial presented by Sean Taylor, Field Application Specialist, Bio-Rad Laboratories, you will learn the basics of high resolution melt analysis and how to practically use it in your research. The video contains information on:
- what is high resolution melt analysis, how does it work and why it is useful
- various applications for high resolution melt analysis including single nucleotide polymorphism (SNP) analysis and DNA methylation analysis
- the difference between a standard melt curved performed in routine QPCR and HRM
- the difference between non-saturation dyes such as SYBR green and saturation dyes such as evagreen
- the definition of the four class types of SNPS
- critical factors for good experimental design
- what technique you should be using for your SNP analysis (it is not one size fits all…think HRM VS Sequenome VS multiplex array
- and more!!!
For more information on HRM see A Practical Guide to High Resolution Melt Analysis Genotyping