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Explaining Cancer Through Narrated Cartoons

 :: Posted by American Biotechnologist on 08-16-2011

The New York Times has a great cartoon video explaining three predominant theories of how cancer progresses from a single cell into a metastatic tumor. The narrated video discusses:

  1. The microbe theory-whereby an otherwise symbiotic relationship between bacteria and their host is spoiled by miscommunication
  2. The Junk DNA theory
  3. The MicroRNA theory-whereby microRNA either prevents mRNA from reaching the ribosome or intercepts and changes the mRNA message

Click on the picture below to see the video on the NYT website.

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No Comments » | Videos

7 Great Resources for HRM Analysis

 :: Posted by American Biotechnologist on 08-04-2011

Bio-Rad Laboratories recently launched the Precision Melt Supermix, which is a high-perfomance supermix for both genotyping and epigenetic analyses.

In honor of this launch, we invite you to review some of the resources (including technical notes, review articles and video tutorials) that we have posted on high resolution melt analysis. Feel free to to click on any of the links below for further details:

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No Comments » | Bio-Rad Tutorial, Bio-Rad Webinar, PCR Video Tutorials, qPCR tutorial, Technical Note, Videos

A video dedicated to all science graduate students

 :: Posted by American Biotechnologist on 01-23-2011

It took me 3 years to complete a 2 year Master’s degree. Why? Because I got stuck with someone else’s poorly planned excuse of a project. My PI promised that if I carefully executed the protocol my first authored paper would be “in the bag.” Have you ever done a DNA gel shift assay? If you have, you know that nothing can be further from the truth than an “in the bag” promise.

This video submission to the Molecular and Human Genetics Retreat 2011 at Baylor College of Medicine totally resonates with me. Thanks to Postdoc Forum for pointing it out!

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No Comments » | just for fun, Videos

A Video Tutorial for High Resolution Melt Analysis

 :: Posted by American Biotechnologist on 01-10-2011

High resolution melt (HRM) analysis is a relatively new technique used in detecting small variations in DNA sequences between varying populations. Important applications of HRM include SNP analysis, genotyping and methylation analysis.

In the following 20 minute tutorial presented by Sean Taylor, Field Application Specialist, Bio-Rad Laboratories, you will learn the basics of high resolution melt analysis and how to practically use it in your research. The video contains information on:

  • what is high resolution melt analysis, how does it work and why it is useful
  • various applications for high resolution melt analysis including single nucleotide polymorphism (SNP) analysis and DNA methylation analysis
  • the difference between a standard melt curved performed in routine QPCR and HRM
  • the difference between non-saturation dyes such as SYBR green and saturation dyes such as evagreen
  • the definition of the four class types of SNPS
  • critical factors for good experimental design
  • what technique you should be using for your SNP analysis (it is not one size fits all…think HRM VS Sequenome VS multiplex array
  • and more!!!

For more information on HRM see A Practical Guide to High Resolution Melt Analysis Genotyping

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No Comments » | Bio-Rad Tutorial, Protocols, qPCR tutorial, Videos

Real Time Quantitative PCR Software Tutorial

 :: Posted by American Biotechnologist on 01-04-2011

If you are a user or potential users of Bio-Rad’s CFX or MiniOpticon systems be sure to see the message below and the accompanying real time quantitative PCR software tutorial from Bio-Rad’s Sean Taylor, Field Applications Specialist.

I am pleased to provide our recently upgraded version of CFX Manager. Version 2.0 has significant improvements and new tools from the last version 1.6 of the software. I recently did a little test drive and have recorded a 25-minute training video to help you navigate around the new features. Some key highlights include:

1. Scheduling module so that you can schedule your time on the instrument. A great tool for a multi-user instrument.
2. Master Mix Calculator…Just type in your primer concentrations and you get the volumes required for your experiment. No more thinking and more importantly…no more mistakes in volume conversions!
3. Copy and paste wells in the plate editor. I am personally very pleased with this feature.
4. Much easier access to the data you need and very nice and new data view and data export features.

The link to the video is here:
video link

The specifications for the software can be found here:
software specifications

To access the upgrade, you can go to:
upgrade

Click on “Downloads” and you will find:

A. The CFX96 and CFX384 Real-Time PCR Detection Systems Firmware Update.

B. The upgrade patch for CFX Manager 2.0.

C. Precision Melt Analysis™ v1.1 Software Upgrade (This is only required for those who are using Version 1.0 of Precision Melt Analysis Software).

Important: The firmware update comes with detailed instructions that are easy to follow but do not hesitate to call tech support (1-800-4Bio-Rad) if you have any questions about the procedure.

I would like to take this opportunity to wish you and your families health and happiness in 2011!

Sean Taylor
Field Application Specialist
Bio-Rad Laboratories

Click here for other technical resources from Sean including his video entitled “A Practical Approach to MIQE for the Bench Scientist,” A Practical Guide to High Resolution Melt Analysis Genotyping, and the article A Simple Solution to Chromatography for High-Purity Protein Preparations: The Modular Approach

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No Comments » | Bio-Rad Tutorial, qPCR tutorial, Videos
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