Very funny piece from a very funny man. Listener’s discretion is advised.
Archive for the ‘Uncategorized’ Category
The following videos help explain:
1. What is the Nogoya Protocol
2. Why is the Nogoya Protocol necessary?
Bio-Rad Launches New TGX™ FastCast™ and TGX Stain-Free™ FastCast™ Acrylamide Kits for Gel Electrophoresis:: Posted by American Biotechnologist on 10-29-2013
Bio-Rad Laboratories announced the launch of its TGX FastCast acrylamide and TGX Stain-Free FastCast acrylamide kits, which provide handcast gel users access to Bio-Rad’s TGX gel chemistry, previously available only in precast gels. The TGX FastCast kits allow users to hand cast polyacrylamide gels with fast run times, efficient protein transfers, and consistent, reproducible results. Researchers can also take advantage of the TGX Stain-Free version, which allows users to monitor the success of each step in their protein electrophoresis and western blotting workflows.
Fast Casting, Running, and Transfer Times, Long Shelf Life
Unlike traditional handcast gels, which require 60 minutes to polymerize before running, FastCast gels can be used only 30 minutes after casting, saving valuable preparation time. Protein electrophoresis can then be run on these gels in as little as 20 minutes, three times faster than conventional Tris-HCl handcast gels.
As with Bio-Rad’s TGX precast gels, proteins can be transferred from FastCast gels in as little as 3 minutes when used with Bio-Rad’s Trans-Blot® Turbo™ transfer system. In addition to these performance advantages, FastCast gels can conveniently be stored for up to 4 weeks after casting, allowing researchers to cast multiple gels at once and use as needed.
“Having a 4-week shelf life is extremely useful, as the handcast gels I previously worked with only lasted two to three days, forcing me to plan my experiments around this limited shelf life,” said Aaron Ramsey, a graduate student in the biochemistry department at Virginia Tech. “Now I can cast several gels at once that I know will last for several weeks.”
Benefits of Going Stain-Free
The TGX Stain-Free FastCast acrylamide kits allow protein detection in gels or on membranes in 3–5 minutes using Bio-Rad’s stain-free imaging systems. This makes it possible for researchers to cast their gels and achieve complete protein separation, gel imaging, and data analysis in less than an hour.
The importance of stain-free technology is readily apparent in western blotting experiments, where it provides confidence at each step of the workflow through visual checks. Stain-free visualization also enables total protein normalization, a more accurate method than using housekeeping proteins for correcting loading errors.
“The TGX Stain-Free FastCast acrylamide kits allow me to skip using extra antibodies for detecting loading controls because I can use a stain-free gel image for normalization to multiple proteins in the sample,” said Alan Cochrane, PhD, a professor in the Department of Molecular Genetics at the University of Toronto.
Dr. Cochrane also says that stain-free gels give him better sensitivity than Coomassie staining.
To learn more about the TGX FastCast and TGX Stain-Free FastCast acrylamide kits, visit www.bio-rad.com/FastCast-acrylamide.
After failing to enact legislation on the 2014 fiscal budget, the US government shutdown and furloughed approximately 800,000 federal workers. For seventeen nerve-racking days in October, (October 1-17), many routine government services were closed, costing the economy a whopping $24 Billion dollars. While the shutdown affected many public services and universities, it had a particularly crippling effect on one of America’s largest research institutes, the National Institute of Health. Despite the fact that the shutdown ended several days ago, many scientists continue to wonder what long-term impact the fiasco will have on their funding. Considering that the NIH supports more than 50% of American research projects, this question is completely justified.
In an article posted to the NIH website on October 17th, Sally Rockey, the NIH’s Deputy Director for Extramural Research, inforomed scientists that October grant submission deadlines will be rescheduled to November and missed reviews may or may not be rescheduled depending on the particular reviewer’s personal situation.
How do scientists feel about this setback? From the comments posted to the NIH blog, not very happy.
One PI wonders if it is even worth applying for NIH funding for the coming year. He correctly states that although the shutdown has ended, the sequester continues to loom large and hasn’t left much hope for scientists looking for grants in 2014. Even more worryingly, the commenter tells us of the impact that the shutdown/sequester has had on the next generation of American scientists which his best junior scientist moving to Malta where she has been promised more lucrative scientific funding.
Another scientists worries that:
five month delay in grant reviews may be career-ending for many scientists
Or as another scientist wrote:
Years of preparations by researchers who’s careers are dependent on these reviews will be put in peril because the agency closed for a couple of weeks
Will American science ever recover from these setbacks? How long can we sustain our position as a world leader in scientific discovery? If the responses from NIH scientists are any indication, we are entering a deep dark tunnel and it may take us years to dig out.
In previous posts we covered 8 metrics to define scientific success and a full length video of Darren Griffin’s ten commandments for becoming a successful scientist. In the post below, we’ve written out these comandments for those readers who may not have 54 minutes to spare watching the full talk.
- There is only one way to do good research: get on with it!
- When opportunity knocks, open the door
- Build a team of people that are better than you are
- It’s not about your knowledge. It’s about your imagination, ideas and talented friends
- Always bring something to the party when collaborating (don’t forget it’s “give and take”)
- It’s not the size of your gun-it’s when you shoot. Timing is everything.
- If the systen doesn’t work for you-change it, do something else, or don’t complain! Nobody likes a winer
- Don’t ask why. Ask why not. How can you improve? Don’t take no for an answer right away when your grant or paper is rejected. Every no is one step closer to a yes! Learn how to turn rejection into an opportunity.
- The journey is often far more rewarding than the destination.
- Be nice to people! What goes around comes around.
What are your thoughts about Griffin’s commandments?